Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography
| Autor: | W H Civin, S Weiner, M D Kolins, E Epstein |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Phosphatidylglycerol
Phosphatidylethanolamine Chromatography Amniotic fluid food.ingredient Biochemistry (medical) Clinical Biochemistry technology industry and agriculture Phosphatidylserine Lecithin Thin-layer chromatography chemistry.chemical_compound food chemistry Phosphatidylcholine lipids (amino acids peptides and proteins) Sphingomyelin |
| Zdroj: | Clinical Chemistry. 26:403-405 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/26.3.403 |
| Popis: | We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|