Effects of natural honey on polymicrobial culture of various human pathogens
| Autor: | Ahmad Al Khazim Al Ghamdi, Faiza S Al-Waili, Amjed Ali, Noori S. Al-Waili, Mohammed Akmal, Khelod Salom |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Staphylococcus aureus
animal structures Streptococcus pyogenes Human pathogen honey Bacterial growth medicine.disease_cause Microbiology Candida albicans medicine Escherichia coli biology business.industry fungi digestive oral and skin physiology food and beverages General Medicine Antimicrobial biology.organism_classification Corpus albicans Basic Research behavior and behavior mechanisms business |
| Zdroj: | Archives of Medical Science : AMS |
| ISSN: | 1896-9151 1734-1922 |
| Popis: | INTRODUCTION Honey has a wide range of antimicrobial activity. All previous studies have considered honey's effect on a single microbe. The present study investigated activity of honey towards a high dose of single or polymicrobial culture. MATERIAL AND METHODS 10 µl specimens of Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli) and Candida albicans (C. albicans) were cultured in 10 ml of 10-100% (wt/v) honey diluted in broth. Six types of polymicrobial microbial cultures were prepared by culturing the isolates with each other onto broth (control) and broth containing various concentrations of honey (10-100% wt/v). Microbial growth was assessed on solid plate media after 24 h incubation. RESULTS Honey (30-70%) prevents growth of 10 µl specimens of all the isolates. Greater reduction in growth of E. coli was observed when cultured with S. aureus. Culturing of S. aureus with S. pyogenes, C. albicans, or E. coli increased its sensitivity to honey. S. aureus and S. pyogenes increased sensitivity of C. albicans to honey while E. coli and C. albicans decreased sensitivity of S. pyogenes. CONCLUSIONS It might be concluded that honey prevents and inhibits growth of single and polymicrobial pathogenic cultures. Polymicrobial culture affects growth of the isolates and increases their sensitivity to honey. |
| Databáze: | OpenAIRE |
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