Rapid method to screen and sort lipid accumulating microalgae
| Autor: | Maria J. Barbosa, Dorinde M.M. Kleinegris, Iago Teles Dominguez Cabanelas, René H. Wijffels, Mathijs van der Zwart |
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| Rok vydání: | 2014 |
| Předmět: |
Boron Compounds
Bio Process Engineering Environmental Engineering Cell Survival Bioengineering biodiesel Cell Separation Biology Cellular viability strains Fluorescence-Activated Cell Sorting chemistry.chemical_compound Chlorococcum littorale Oxazines Microalgae Waste Management and Disposal VLAG Nannochloropsis sp Renewable Energy Sustainability and the Environment nile red Cellular lipid Nile red General Medicine Lipids biofuels Staining BBP Bioconversion fluorescence method Biochemistry chemistry fatty-acid profile nannochloropsis sp Biotechnology |
| Zdroj: | Bioresource Technology 184 (2015) Bioresource Technology, 184, 47-52 |
| ISSN: | 1873-2976 0960-8524 |
| Popis: | The present work established an efficient staining method for fluorescence activated cell sorting (FACS) with Chlorococcum littorale maintaining cellular viability. The method was designed to detect high-lipid cells and to guarantee cellular viability. BODIPY505/515 (BP) was more suitable to FACS when compared to Nile red. The optimum concentrations were 0.4 μg ml−1 of BP, 0.1% DMSO or 0.35% ethanol. Both ethanol and DMSO were equally efficient and assured cellular viability after the staining and sorting. Here a method is presented to rapidly screen and sort lipid rich cells of C. littorale with FACS, which can be used to produce new inoculum with increased cellular lipid content. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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