Nucleus Accumbens Chemogenetic Inhibition Suppresses Amphetamine-Induced Ultrasonic Vocalizations in Male and Female Rats
Autor: | Christina M. Ruiz, Abigail Y Flores, Rachael E. Hokenson, Kate A Lawson, Stephen V. Mahler |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Agonist
Subjective effects medicine.drug_class nucleus accumbens males amphetamine Neurosciences. Biological psychiatry. Neuropsychiatry Nucleus accumbens Behavioral neuroscience Biology Basic Behavioral and Social Science Article clozapine-n-oxide Long evans rats Behavioral and Social Science medicine Psychology Amphetamine 50 kHz vocalizations 22 kHz vocalizations Human studies kHz vocalizations General Neuroscience Neurosciences UMAP Chemogenetics females Brain Disorders Cognitive Sciences chemogenetics Neuroscience medicine.drug RC321-571 |
Zdroj: | Brain Sciences, Vol 11, Iss 1255, p 1255 (2021) Brain sciences, vol 11, iss 10 Brain Sciences Volume 11 Issue 10 |
ISSN: | 2076-3425 |
Popis: | Adult rats emit ultrasonic vocalizations (USVs) related to their affective states, potentially providing information about their subjective experiences during behavioral neuroscience experiments. If so, USVs might provide an important link between invasive animal preclinical studies and human studies in which subjective states can be readily queried. Here, we induced USVs in male and female Long Evans rats using acute amphetamine (2 mg/kg), and asked how reversibly inhibiting nucleus accumbens neurons using designer receptors exclusively activated by designer drugs (DREADDs) impacts USV production. We analyzed USV characteristics using “Deepsqueak” software, and manually categorized detected calls into four previously defined subtypes. We found that systemic administration of the DREADD agonist clozapine-n-oxide, relative to vehicle in the same rats, suppressed the number of frequency-modulated and trill-containing USVs without impacting high frequency, unmodulated (flat) USVs, nor the small number of low-frequency USVs observed. Using chemogenetics, these results thus confirm that nucleus accumbens neurons are essential for production of amphetamine-induced frequency-modulated USVs. They also support the premise of further investigating the characteristics and subcategories of these calls as a window into the subjective effects of neural manipulations, with potential future clinical applications. |
Databáze: | OpenAIRE |
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