A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A
| Autor: | Rebecca J. M. Goss, Maoluo Gan, Matthias Onyebuchi Agbo, P. Andrew Evans, Grahame Mackenzie, Emily R. Abraham, Refaat B. Hamed, Alberto Diego-Taboada, Christopher S. Bailey, Hong Gao, Joseph S. Zarins-Tutt |
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| Přispěvatelé: | European Research Council, University of St Andrews. School of Chemistry, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. EaSTCHEM |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Lycopodium clavatum
biology 010405 organic chemistry medicine.drug_class Antibiotics NDAS General Chemistry 010402 general chemistry biology.organism_classification Polyene QD Chemistry 01 natural sciences Combinatorial chemistry 0104 chemical sciences chemistry.chemical_compound Sporopollenin chemistry Direct exposure Photoprotection medicine Light sensitive QD BDC |
| Popis: | This work was supported by an MRC Case Studentship (JZT, RJMG), the European Research Council, funded under the European Union’s Seventh Framework Programme (FP7/2007-2013/ERC consolidator grant GCGXC grant agreement no 614779 to RJMG). We also gratefully acknowledge a CSC Scholarship (MG, RJMG), a BSAC Fellowship (MA, RJMG), and Sporomex Ltd (ADT) for supporting this work. NSERC is thanked for supporting a Tier 1 Canada Research Chair (PAE) and the Royal Society for a Wolfson Research Merit Award (PAE). We also thank W. Fenical (Scripps Institution of Oceanography, San Diego) for the kind provision of Marinispora strain CNQ-140. The photoprotection and isolation of marinomycin A using sporopollenin exine capsules (SpECs) derived from the spores of the plant Lycopodium clavatum is described. The marinomycins have a particularly short half-life in natural light, which severely impacts their potential biological utility given that they display potent antibiotic and anticancer activity. The SpEC encapsulation of the marinomycin A dramatically increases the half-life of the polyene macrodiolide to the direct exposure to UV radiation by several orders of magnitude, thereby making this a potentially useful strategy for other light sensitive bioactive agents. In addition, we report that the SpECs can also be used to selectively extract culture broths that contain the marinomycins, which provides a significantly higher recovery than with conventional XAD resins and provides concomitant photoprotection. Publisher PDF |
| Databáze: | OpenAIRE |
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