Porcine model of progressive cardiac hypertrophy and fibrosis with secondary postcapillary pulmonary hypertension

Autor: Zsuzsanna Szankai, Jutta Bergler-Klein, Dominika Lukovic, Andreas Spannbauer, Mariann Gyöngyösi, Andras Jakab, Denise Traxler, Rita Garamvölgyi, Sandor Batkai, Johannes Winkler, Frederic Jaisser, Faiez Zannad, Katrin Zlabinger, Thomas Thum, Noemi Pavo, Ljubica Mandic, Alfred Gugerell, Gerald Maurer, Georg Goliasch, Levente Tóth
Přispěvatelé: BMC, BMC, Department of Cardiology [Vienna, Austria], Medizinische Universität Wien = Medical University of Vienna, Institute of Diagnostic Imaging and Radiation Oncology [Kaposvar, Hungary], University of Kaposvar [Hungary], Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'investigation clinique [Nancy] (CIC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Institute for Molecular and Translational Therapeutic Strategies - IMTTS [Hannover, Germany], Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Medical University of Vienna, Centre de Recherche des Cordeliers ( CRC ), Université Paris Diderot - Paris 7 ( UPD7 ) -École pratique des hautes études ( EPHE ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), CIC-Nancy, Institut Lorrain du Coeur et des Vaisseaux Louis Mathieu [Nancy]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), University of Zurich, Gyöngyösi, Mariann
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
[SDV]Life Sciences [q-bio]
Sus scrofa
lcsh:Medicine
Hemodynamics
030204 cardiovascular system & hematology
0302 clinical medicine
Diastole
Ejection fraction
medicine.diagnostic_test
Aortic isthmus stenosis
Translational large animal model
General Medicine
3. Good health
Gene Expression Regulation
Neoplastic
[SDV] Life Sciences [q-bio]
Cardiac hypertrophy
medicine.anatomical_structure
Disease Progression
Cardiology
medicine.medical_specialty
Aortography
Systole
Heart Ventricles
Hypertension
Pulmonary
Magnetic Resonance Imaging
Cine
610 Medicine & health
Cardiomegaly
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Afterload
1300 General Biochemistry
Genetics and Molecular Biology
Internal medicine
medicine.artery
Pressure
medicine
Animals
Pressure overload
[ SDV ] Life Sciences [q-bio]
business.industry
Myocardium
Research
lcsh:R
Aortic Valve Stenosis
medicine.disease
Fibrosis
Capillaries
Disease Models
Animal
030104 developmental biology
10036 Medical Clinic
Ventricle
Heart failure
PET-MRI imaging
Pulmonary artery
Gene expression
business
Biomarkers
Zdroj: Journal of Translational Medicine
Journal of Translational Medicine, BioMed Central, 2016, 15 (1), pp.202. ⟨10.1186/s12967-017-1299-0⟩
Journal of Translational Medicine, 2016, 15 (1), pp.202. ⟨10.1186/s12967-017-1299-0⟩
Journal of Translational Medicine, Vol 15, Iss 1, Pp 1-15 (2017)
Journal of Translational Medicine, BioMed Central, 2016, 15 (1), pp.202. 〈10.1186/s12967-017-1299-0〉
ISSN: 1479-5876
DOI: 10.1186/s12967-017-1299-0⟩
Popis: Background Meaningful translational large animal models for cardiac diseases are indispensable for studying disease mechanisms, development of novel therapeutic strategies, and evaluation of potential drugs. Methods For induction of heart failure, cardiac hypertrophy and fibrosis, a bare metal stent was implanted in the descending aorta of growing pigs (n = 7), inducing pressure stress on the left ventricle (group HYPI). The constant stent size in growing pigs resulted in antegrade partial obstruction of the aortic flow with a gradual increase in afterload. Five pigs with sham intervention served as control. Serial haemodynamic, pressure–volume loop measurements and transthoracic echocardiography (TTE) were performed to detect developing pressure overload of the LV and cardiac MRI with late enhancement for measuring LV and RV mass and ejection fraction. Results At 5-month follow-up, CT and contrast aortography, and intraluminal echocardiography confirmed aortic isthmus stenosis with a mean trans-stenotic gradient of 64 ± 13.9 mmHg. Invasive haemodynamic measurements revealed a secondary increase in pulmonary artery pressure (44.6 ± 5.1 vs 25.9 ± 6.2 mmHg, HYPI vs control, p
Databáze: OpenAIRE
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