Effect of GABA, a Bacterial Metabolite, on Pseudomonas fluorescens Surface Properties and Cytotoxicity
| Autor: | Cécile Duclairoir-Poc, Audrey Dagorn, Sylvie Chevalier, Laure Taupin, Marc G. J. Feuilloley, Nicole Orange, Annelise Chapalain, Lily Mijouin, Mélanie Hillion |
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| Přispěvatelé: | Laboratoire de Microbiologie du Froid – Signaux et Micro-Environnement (LMDF-SME), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Pathogenèse des légionelles- Legionella pathogenesis (LegioPath), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), CHEVALIER, Sylvie, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
MESH: Cell Death MESH: gamma-Aminobutyric Acid lcsh:Chemistry Diffusion Lipid A chemistry.chemical_compound MESH: Animals lcsh:QH301-705.5 MESH: GABA-A Receptor Antagonists MESH: Receptors GABA-A gamma-Aminobutyric Acid Spectroscopy 0303 health sciences Pyoverdine Cell Death biology host-microbial interactions cytotoxicity virulence bacterial adhesion lipopolysaccharide biofilms pyoverdine Muscimol GABAA receptor MESH: Diffusion General Medicine Computer Science Applications Biochemistry MESH: Oligopeptides MESH: Neuroglia Neuroglia Oligopeptides medicine.drug MESH: Rats Surface Properties Pseudomonas fluorescens MESH: Biofilms Bicuculline Article Catalysis Microbiology Inorganic Chemistry MESH: Pseudomonas fluorescens 03 medical and health sciences Caseinase MESH: Bicuculline medicine Animals Humans GABA-A Receptor Agonists GABA-A Receptor Antagonists MESH: Bacterial Adhesion MESH: GABA-A Receptor Agonists Physical and Theoretical Chemistry Molecular Biology 030304 developmental biology MESH: Surface Properties MESH: Humans 030306 microbiology Organic Chemistry Biofilm Receptors GABA-A biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Rats lcsh:Biology (General) lcsh:QD1-999 chemistry nervous system MESH: Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Lipopolysaccharides MESH: Muscimol |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, MDPI, 2013, 14 (6), pp.12186-12204. ⟨10.3390/ijms140612186⟩ International Journal of Molecular Sciences, 2013, 14 (6), pp.12186-12204. ⟨10.3390/ijms140612186⟩ International Journal of Molecular Sciences, Vol 14, Iss 6, Pp 12186-12204 (2013) International Journal of Molecular Sciences; Volume 14; Issue 6; Pages: 12186-12204 |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms140612186⟩ |
| Popis: | International audience; Different bacterial species and, particularly Pseudomonas fluorescens, can produce gamma-aminobutyric acid (GABA) and express GABA-binding proteins. In this study, we investigated the effect of GABA on the virulence and biofilm formation activity of different strains of P. fluorescens. Exposure of a psychotropic strain of P. fluorescens (MF37) to GABA (10-5 M) increased its necrotic-like activity on eukaryotic (glial) cells, but reduced its apoptotic effect. Conversely, muscimol and bicuculline, the selective agonist and antagonist of eukaryote GABAA receptors, respectively, were ineffective. P. fluorescens MF37 did not produce biosurfactants, and its caseinase, esterase, amylase, hemolytic activity or pyoverdine productions were unchanged. In contrast, the effect of GABA was associated to rearrangements of the lipopolysaccharide (LPS) structure, particularly in the lipid A region. The surface hydrophobicity of MF37 was marginally modified, and GABA reduced its biofilm formation activity on PVC, but not on glass, although the initial adhesion was increased. Five other P. fluorescens strains were studied, and only one, MFP05, a strain isolated from human skin, showed structural differences of biofilm maturation after exposure to GABA. These results reveal that GABA can regulate the LPS structure and cytotoxicity of P. fluorescens, but that this property is specific to some strains. |
| Databáze: | OpenAIRE |
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