Localization, purification, and characterization of a novel β‐glucosidase from Hanseniaspora uvarum Yun268

Autor:	Tongtong Fan, Siyu Jing, Hongyan Zhang, Xiaobing Yang, Guojie Jin, Yongsheng Tao
Rok vydání:	2022
Předmět:	Hanseniaspora beta-Glucosidase Fermentation Odorants Wine Food Science
Zdroj:	Journal of Food Science. 87:886-894
ISSN:	1750-3841 0022-1147
DOI:	10.1111/1750-3841.16068
Popis:	β-Glucosidase is a key enzyme that hydrolyzes nonvolatile glycosylated precursors of aroma compounds and enhances the organoleptic quality of wines. In this study, a novel β-glucosidase from Hanseniaspora uvarum Yun268 was localized, purified, and characterized. Results indicated that β-glucosidase activity was mainly distributed within the cells. After purification via ammonium sulfate precipitation combined with chromatography, β-glucosidase specific activity increased 8.36 times, and the activity recovery was 56.90%. The enzyme had a molecular mass of 74.22 kDa. It has a Michaelis constant (K
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27bb8c53b6ac2a0086d0970507cc0ac2 https://doi.org/10.1111/1750-3841.16068 Zobrazit plný text záznamu Plný text