Impact of cigarette smoke and IL-17A activation on asthmatic patients with chronic rhinosinusitis
| Autor: | CC Huang, TJ Lee, PH Chang, CH Fu, PW Wu, CH Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
03 medical and health sciences 0302 clinical medicine otorhinolaryngologic diseases medicine Humans Sinusitis 030223 otorhinolaryngology Adverse effect Rhinitis Asthma biology business.industry Interleukin-17 Smoking Innate lymphoid cell Interleukin General Medicine respiratory system Aryl hydrocarbon receptor medicine.disease respiratory tract diseases Cytokine Otorhinolaryngology Immunology biology.protein business Immunostaining |
| Zdroj: | Rhinology journal. 57:57-66 |
| ISSN: | 0300-0729 |
| DOI: | 10.4193/rhin18.131 |
| Popis: | Background: Cigarette smoke have adverse effects in the control of asthma and chronic rhinosinusitis (CRS). Interleukin (IL)-17A, the signature cytokine of helper T 17 cells and group 3 innate lymphoid cells (ILC3), has been reported to link with resistance to therapy in airway inflammation. This study aimed to investigate the impact of cigarette smoking and IL-17A activation on the clinical outcomes of asthmatic patients with chronic rhinosinusitis. Methods: 33 patients with CRS and asthma, including 15 smokers and 18 non-smokers, and 7 asthmatic patients without CRS and smoking were prospectively recruited. The Sino-Nasal Outcome Test-22 and Asthma Control Test were used to evaluate sinonasal symptoms and the level of asthma control, respectively. Real-time PCR and immunostaining were applied to evaluate the expression levels of IL-17A and associated immunological factors on surgically-obtained nasal tissues. Results: Nasal surgery improved both sinonasal symptoms and asthma control. Compared to non-smokers, smokers showed poorer improvement in asthma control. The expression of IL-17A, IL-22, aryl hydrocarbon receptor (AhR), and ILC3 was increased in the nasal tissues of smokers with asthma and CRS. The expression of IL-17A mRNA was correlated with that of AhR and with positive nuclear AhR-AhR nuclear translocator staining cells, and that of cyclooxygenase-2 enzyme (COX-2). ILC3 cells were associated with IL-17A, IL-22, AhR, and COX-2 mRNA expression. Conclusions: Cigarette smoking was related to lesser improvement in asthma control after nasal surgery and to IL-17A activation in the nasal tissues of patients with inflammatory airways. |
| Databáze: | OpenAIRE |
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