Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats

Autor: Natasa Kustrimovic, Iva Aleksić, Katarina Mitić, Stanislava Stanojević, Vesna Vujić, Mirjana Dimitrijević
Rok vydání: 2013
Předmět:
Male
Phagocytosis
Albino Oxford (AO) rat strain
Peritoneal macrophage phenotype
Biology
Nitric Oxide
General Biochemistry
Genetics and Molecular Biology
Cell Degranulation
Nitric oxide
Immunophenotyping
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Species Specificity
Compound 48/80
Macrophage
Animals
p-Methoxy-N-methylphenethylamine
Mast Cells
General Pharmacology
Toxicology and Pharmaceutics
Mast cell degranulation
030304 developmental biology
Dark Agouti (DA) rat strain
CD86
0303 health sciences
Tumor Necrosis Factor-alpha
Zymosan
Degranulation
Peritoneal macrophage functions
General Medicine
Hydrogen Peroxide
Molecular biology
Rats
chemistry
Thioglycollate peritonitis
Thioglycolates
Immunology
Macrophages
Peritoneal
Tumor necrosis factor alpha
030215 immunology
Zdroj: Life Sciences
ISSN: 1879-0631
Popis: Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.
Databáze: OpenAIRE
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