Salivary gland cell aggregates are derived from self-organization of acinar lineage cells
| Autor: | M. Eva Hansen, Jomy J. Varghese, Danielle S. W. Benoit, Matthew H. Ingalls, Martha A. Ormanoski, Catherine E. Ovitt, Azmeer Sharipol, Shawn D. Newlands |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lineage (genetic) Cell Population chemistry.chemical_element Cell Count Acinar Cells Calcium Salivary Glands Article Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system Extracellular medicine Animals Humans education General Dentistry Cells Cultured Cell Proliferation education.field_of_study Salivary gland Cell Differentiation 030206 dentistry Cell Biology General Medicine In vitro Cell biology 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology chemistry Stem cell |
| Zdroj: | Archives of Oral Biology. 97:122-130 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2018.10.017 |
| Popis: | Objective: The objective of this study was to characterize the mechanism by which salivary gland cells (SGC) aggregate in vitro. Design: Timelapse microscopy was utilized to analyze the process of salivary gland aggregate formation using both primary murine and human salivary gland cells. The role of cell density, proliferation, extracellular calcium, and secretory acinar cells in aggregate formation was investigated. Finally, the ability of cells isolated from irradiated glands to form aggregates was also evaluated. Results: Salivary gland cell self-organization rather than proliferation was the predominant mechanism of aggregate formation in both primary mouse and human salivary gland cultures. Aggregation was found to require extracellular calcium while acinar lineage cells account for ∼80% of the total aggregate cell population. Finally, aggregation was not impaired by irradiation. Conclusions: The data reveal that aggregation occurs as a result of heterogeneous salivary gland cell self-organization rather than from stem cell proliferation and differentiation, contradicting previous dogma. These results suggest a re-evaluation of aggregate formation as a criterion defining salivary gland stem cells. |
| Databáze: | OpenAIRE |
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