Food antigen-induced immune responses in Crohn's disease patients and experimental colitis mice

Autor: Yasuyo Suga, Manabu Suzuki, Keiko Saito, Takayuki Kajiura, Kaori Oomura, Masaki Hashimoto, Akira Mitsui, Junko Shinozaki, Maiko Mori, Yuka Ikenoue, Keiko Danjo, Masakazu Takazoe, Takaaki Kawaguchi, Naoki Yoshimura, Michihide Uo, Minako Sako
Rok vydání: 2014
Předmět:
CD4-Positive T-Lymphocytes
Male
Original Article—Alimentary Tract
Crohn’s disease
animal diseases
Disease
Lymphocyte Activation
Crohn Disease
Surgical oncology
Cells
Cultured
Mice
Knockout
Crohn's disease
Mice
Inbred BALB C
digestive
oral
and skin physiology
Gastroenterology
Middle Aged
Colitis
Experimental colitis
Interleukin-10
Female
Food Hypersensitivity
Adult
medicine.medical_specialty
Adolescent
Antigen-Presenting Cells
chemical and pharmacologic phenomena
Young Adult
CD4+ T cell
Immune system
Antigen
Food antigens
Internal medicine
medicine
Animals
Humans
Antigens
Aged
business.industry
Serum immunoglobulin G
Case-control study
biochemical phenomena
metabolism
and nutrition
Hepatology
medicine.disease
Disease Models
Animal
Case-Control Studies
Immunoglobulin G
Immunology
bacteria
Colitis
Ulcerative
business
Zdroj: Journal of Gastroenterology
ISSN: 1435-5922
Popis: Background In Crohn’s disease (CD), the involvement of food antigens in immune responses remains unclear. The objective of this study was to detect immune responses against food antigens in CD patients and examine the mechanism in a mouse model of colitis. Methods We enrolled 98 CD patients, 50 ulcerative colitis patients, and 52 healthy controls (HCs) to compare the levels of serum immunoglobulin (Ig)Gs against 88 foods. The presence of serum IgGs against foods was also examined in interleukin (IL)-10 knockout (KO) mice in which CD4+ T cell activation by antigenic food protein was assessed. Mice transferred with IL-10 KO cells received diets with or without food antigens, and the development of colitis was evaluated. Results The prevalence of IgGs against various foods, especially vegetables, grains, and nuts, was significantly higher in CD patients than in HCs. Similarly, the prevalence of IgGs against food proteins was higher in IL-10 KO mice than in BALB/c mice. Beta-conglycinin, identified as an antigenic food proteins in IL-10 KO mice, induced CD4+ T cell production of interferon-γ and IL-17 through dendritic cell antigen presentation. Elimination of the food antigens ameliorated the development of colitis in mice without altering the composition of their intestinal microbiota. Conclusions In CD colitis mice, intestinal inflammation via CD4+ T cell hyperactivation was induced by food antigens associated with high serum IgG levels and was ameliorated by the elimination of food antigens. This disrupted immunological tolerance to food antigen, which might act as an exacerbating factor, remains to be elucidated in CD patients. Electronic supplementary material The online version of this article (doi:10.1007/s00535-014-0981-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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