Analysis of fatty acid methyl esters with high accuracy and reliability
| Autor: | Cecil D. Bannon, Desdemona E. Albertyn, Ngo Trong Hai, Charlotte Szonyi, Kerry L. O'Rourke, John D. Craske |
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| Rok vydání: | 1982 |
| Předmět: |
Response factor
chemistry.chemical_classification Analyte Chromatography Aqueous solution food.ingredient Hydrogen Detector Organic Chemistry Extraction (chemistry) Coconut oil Linearity chemistry.chemical_element Fatty acid General Medicine Methoxide Oxygen Biochemistry Analytical Chemistry chemistry.chemical_compound food Linear range chemistry Organic chemistry |
| Zdroj: | Journal of Chromatography A. 247:71-89 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)84857-8 |
| Popis: | The literature leading to the development of rapid procedures for the preparation of fatty acid methyl esters for gas—liquid chromatography by methoxide-catalysed methanolysis of fats and oils is reviewed. A rapid and reliable procedure utilizing the best features of the literature methods was developed and shown to give excellent quantitative results in the difficult case of a triacylglycerol primary standard mixture simulating coconut oil. Evidence is presented that brief refluxing of the reaction mixture is advantageous and that removal of methoxide by aqueous extraction at the completion of the reaction, as distinct form injecting the whole reaction mixture, is essential if good quantitative resutls are to be obtained. The method is faster and simpler than the existing international procedures, and the ester solution may be ready for injection into the chromatograph in about 2 min. |
| Databáze: | OpenAIRE |
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