Micro-matrix solid-phase dispersion coupled with MEEKC for quantitative analysis of lignans in Schisandrae Chinensis Fructus using molecular sieve TS-1 as a sorbent
| Autor: | Jun Cao, Mengmeng Wei, Chu Chu, Liqiong Zheng, Zheng He, Jizhong Yan, Shan Wang |
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| Rok vydání: | 2017 |
| Předmět: |
Sorbent
Clinical Biochemistry Analytical chemistry 01 natural sciences Biochemistry Dispersant Sensitivity and Specificity Lignans Analytical Chemistry Matrix (chemical analysis) Surface-Active Agents Microemulsion Chromatography Micellar Electrokinetic Capillary Schisandra Titanium Chromatography 010405 organic chemistry Elution Chemistry Plant Extracts Methanol Silicates 010401 analytical chemistry Extraction (chemistry) Solid Phase Extraction Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Solvent Fruit Linear Models Dispersion (chemistry) |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1063 |
| ISSN: | 1873-376X |
| Popis: | A simple and effective method was developed for determining lignans in Schisandrae Chinensis Fructus by using a micro-matrix solid phase dispersion (MSPD) technique coupled with microemulsion electrokinetic chromatography (MEEKC). Molecular sieve, TS-1, was applied as a solid supporting material in micro MSPD extraction for the first time. Parameters that affect extraction efficiency, such as type of dispersant, mass ratio of the sample to the dispersant, grinding time, elution solvent and volume were optimized. The optimal extraction conditions involve dispersing 25 mg of powdered Schisandrae samples with 50 mg of TS-1 by a mortar and pestle. A grinding time of 150 s was adopted. The blend was then transferred to a solid-phase extraction cartridge and the target analytes were eluted with 500 μL of methanol. Moreover, several parameters affecting MEEKC separation were studied, including the type of oil, SDS concentration, type and concentration of cosurfactant, and concentration of organic modifier. A satisfactory linearity (R > 0.9998) was obtained, and the calculated limits of quantitation were less than 2.77 μg/mL. Finally, the micro MSPD-MEEKC method was successfully applied to the analysis of lignans in complex Schisandrae fructus samples. |
| Databáze: | OpenAIRE |
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