Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein
| Autor: | Ji Young Mun, Oh Suk Kwon, Ki Sun Kwon, Min Sun Sung, Doo-Byoung Oh |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Recombinant Fusion Proteins
Molecular Sequence Data Muscle Fibers Skeletal Biophysics Intracellular Space Muscle disorder Biology MyoD Muscle Development Protein Engineering Biochemistry Myoblasts Mice MyoD Protein Transduction Genetic Myosin Myocyte Animals Humans Amino Acid Sequence Molecular Biology Cell Nucleus Myogenesis Stem Cells Cell Differentiation Cell Biology musculoskeletal system Molecular biology Coculture Techniques Protein Transport Adipose Tissue Solubility Stem cell Peptides tissues C2C12 |
| Zdroj: | Biochemical and biophysical research communications. 437(1) |
| ISSN: | 1090-2104 |
| Popis: | Human adipose-derived stem cells (hASCs) have great potential as cell sources for the treatment of muscle disorders. To provide a safe method for the myogenic differentiation of hASCs, we engineered the MyoD protein, a key transcription factor for myogenesis. The engineered MyoD (MyoD-IT) was designed to contain the TAT protein transduction domain for cell penetration and the membrane-disrupting INF7 peptide, which is an improved version of the HA2 peptide derived from influenza. MyoD-IT showed greatly improved nuclear targeting ability through an efficient endosomal escape induced by the pH-sensitive membrane disruption of the INF7 peptide. By applying MyoD-IT to a culture, hASCs were efficiently differentiated into long spindle-shaped myogenic cells expressing myosin heavy chains. Moreover, these cells differentiated by an application of MyoD-IT fused to myotubes with high efficiency through co-culturing with mouse C2C12 myoblasts. Because internalized proteins can be degraded in cells without altering the genome, the myogenic differentiation of hASCs using MyoD-IT would be a safe and clinically applicable method. |
| Databáze: | OpenAIRE |
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