Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System
| Autor: | Jun Guo, Ke Wang, Yue Yong, Wen-Xiong Zhou, Jia Zhou, Tong-Yu Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Surgical stress Electroacupuncture medicine.drug_class T-Lymphocytes medicine.medical_treatment Intraperitoneal injection Population (+)-Naloxone Mice Opioid receptor Internal medicine medicine Animals Pharmacology (medical) education education.field_of_study business.industry General Medicine T lymphocyte Analgesics Opioid Mice Inbred C57BL Endocrinology Complementary and alternative medicine business Acupuncture Points CD8 |
| Zdroj: | Chinese Journal of Integrative Medicine. 27:98-105 |
| ISSN: | 1993-0402 1672-0415 |
| DOI: | 10.1007/s11655-020-3158-8 |
| Popis: | To investigate the action mechanisms of electroacupuncture (EA) on postoperative immunosuppression. Male C57BL/6 mice (5`-7 weeks old) were randomly divided into: the sham injury group, the surgical trauma stressed group, the EA group [surgery + 2/100 Hz EA at Neiguan (PC 6)], and the EA+ Nal (surgery + EA + intraperitoneal injection of naloxone). Abdominal surgical trauma stress mice model was established. EA was performed on bilateral PC 6 acupoints by an EA apparatus (2/100 Hz) for 20 min once a day for 3 days. The mRNA expressions of MOR, DOR, and KOR in thymus and L3`-L5 dorsal root ganglions (DRG) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and the protein expressions of MOR, DOR, and KOR in thymus were measured by Western blot. Flow cytometry assay was used to detect the levels of T lymphocyte subtypes in the peripheral blood. Surgical trauma induced decreased the mRNA expression level of MOR in both thymus (P 0.05). Furthermore, T lymphocyte population of CD3+ and CD4+ was decreased in the peripheral blood after surgical trauma (both P |
| Databáze: | OpenAIRE |
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