Target-specific cellular uptake of PLGA nanoparticles coated with poly(L-lysine)-poly(ethylene glycol)-folate conjugate
| Autor: | Tae Gwan Park, Ji Hoon Jeong, Sun Hwa Kim, Ki Woo Chun |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Surface Properties
Nanoparticle Receptors Cell Surface macromolecular substances Gene delivery Sensitivity and Specificity Polyethylene Glycols chemistry.chemical_compound Structure-Activity Relationship Drug Delivery Systems Folic Acid Polylactic Acid-Polyglycolic Acid Copolymer Cell Line Tumor PEG ratio Polymer chemistry Electrochemistry Humans General Materials Science Polylysine Lactic Acid Particle Size Spectroscopy Molecular Structure X-Rays Folate Receptors GPI-Anchored technology industry and agriculture Surfaces and Interfaces Condensed Matter Physics Flow Cytometry Glycolates PLGA chemistry Folate receptor Spectrophotometry Drug delivery Biophysics Nanoparticles Carrier Proteins Ethylene glycol Polyglycolic Acid Conjugate |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 21(19) |
| ISSN: | 0743-7463 |
| Popis: | Poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles with anionic surface charge were surface coated with cationic di-block copolymer, poly(L-lysine)-poly(ethylene glycol)-folate (PLL-PEG-FOL) conjugate, for enhancing their site-specific intracellular delivery against folate receptor overexpressing cancer cells. The PLGA nanoparticles coated with the conjugate were characterized in terms of size, surface charge, and change in surface composition by XPS. By employing the flow cytometry method and confocal image analysis, the extent of cellular uptake was comparatively evaluated under various conditions. PLL-PEG-FOL coated PLGA nanoparticles demonstrated far greater extent of cellular uptake to KB cells, suggesting that they were mainly taken up by folate receptor-mediated endocytosis. The enhanced cellular uptake was also observed even in the presence of serum proteins, possibly due to the densely seeded PEG chains. The PLL-PEG-FOL coated PLGA nanoparticles could be potentially applied for cancer cell targeted delivery of various therapeutic agents. |
| Databáze: | OpenAIRE |
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