Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor
| Autor: | Brian Scott Ferguson, H. Tom Soh, James Swensen, Arica A. Lubin, Yi Xiao, Rebecca Y. Lai, Alan J. Heeger, Kevin W. Plaxco |
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| Rok vydání: | 2009 |
| Předmět: |
Serum
Analyte Optics and Photonics Time Factors Aptamer Microfluidics Nanotechnology Biosensing Techniques DNA Aptamers Electrochemistry Biochemistry Signal Catalysis Article Colloid and Surface Chemistry Blood serum Cocaine Humans Dose-Response Relationship Drug Chemistry General Chemistry DNA Equipment Design Aptamers Nucleotide Microfluidic Analytical Techniques Biosensor |
| Zdroj: | Journal of the American Chemical Society. 131(12) |
| ISSN: | 1520-5126 |
| Popis: | The development of a biosensor system capable of continuous, real-time measurement of small-molecule analytes directly in complex, unprocessed aqueous samples has been a significant challenge, and successful implementation has been achieved for only a limited number of targets. Toward a general solution to this problem, we report here the Microfluidic Electrochemical Aptamer-based Sensor (MECAS) chip wherein we integrate target-specific DNA aptamers that fold, and thus generate an electrochemical signal, in response to the analyte with a microfluidic detection system. As a model, we demonstrate the continuous, real-time (approximately 1 min time resolution) detection of the small-molecule drug cocaine at near physiological, low micromolar concentrations directly in undiluted, otherwise unmodified blood serum. We believe our approach of integrating folding-based electrochemical sensors with miniaturized detection systems may lay the groundwork for the real-time, point-of-care detection of a wide variety of molecular targets. |
| Databáze: | OpenAIRE |
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