Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus
| Autor: | Man Hee Rhee, Lauren Togonon Arayan, Wongi Min, Moon Her, Masahisa Watarai, Hu Jang Lee, Hong Hee Chang, Suk Kim, Alisha Wehdnesday Bernardo Reyes, Jin Ju Lee, Hannah Leah Tadeja Simborio, Huynh Tan Hop |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Endosome animal diseases Phagocytosis B. abortus Brucella abortus Endosomes Platelet Membrane Glycoproteins complex mixtures Microbiology Brucellosis Receptors G-Protein-Coupled Mice 03 medical and health sciences 0302 clinical medicine Animals Macrophage Secretion Platelet-activating factor receptor Janus kinase 2 biology Macrophages Janus Kinase 2 bacterial infections and mycoses Actin cytoskeleton Actin Cytoskeleton RAW 264.7 Cells 030104 developmental biology JAK2 Host-Pathogen Interactions biology.protein Signal transduction Signal Transduction Research Article 030215 immunology |
| Zdroj: | BMC Microbiology |
| ISSN: | 1471-2180 |
| Popis: | Background Brucella abortus is an intracellular pathogen which can infect and persist in host cells through multiple interactions. Above all, its interaction to host cell receptor is important to understand the pathogenic mechanisms of B. abortus. Accordingly, we demonstrated that platelet-activating factor receptor (PAFR) affects host cell response against B. abortus infection. Results First of all, B. abortus infection to macrophage induces secretion of platelet-activating factor (PAF), which is a PAFR agonist. The stimulation of PAFR by PAF remarkably increases B. abortus uptake into macrophages. It induces Janus kinase 2 (JAK2) and p38α phosphorylation, indicating that PAFR-mediated activation of JAK2 signaling leads to enhanced uptake of B. abortus. Moreover, the dynamics of F-actin polymerization revealed that PAFR-mediated B. abortus uptake is related with the reorganization of F-actin and JAK2. Upon B. abortus phagocytosis, reduced PAFR in the membrane and subsequently increased levels of PAFR colocalization with endosomes were observed which indicate that B. abortus uptake into macrophages allowed PAFR trafficking to endosomes. Conclusions This study demonstrated that PAFR has a compelling involvement in B. abortus uptake as a promoter of phagocytosis, which is associated with JAK2 activation. Thus, our findings establish a novel insight into a receptor-related phagocytic mechanism of B. abortus. Electronic supplementary material The online version of this article (doi:10.1186/s12866-016-0685-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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