Thyroid Hormone Modulates Apolipoprotein-AI Gene Expression at the Post-Transcriptional Level in Hep G2 Cells
| Autor: | Yves Vandenbrouck, Marise Mangeney-Andreani, Claude Loriette, Gilbert Bereziat, Brigitte Janvier |
|---|---|
| Přispěvatelé: | Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Transcription Genetic [SDV]Life Sciences [q-bio] 030209 endocrinology & metabolism 030204 cardiovascular system & hematology Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine Gene expression medicine Protein biosynthesis Tumor Cells Cultured Humans RNA Messenger RNA Processing Post-Transcriptional ComputingMilieux_MISCELLANEOUS 030304 developmental biology Regulation of gene expression 0303 health sciences Messenger RNA Thyroid hormone receptor Triiodothyronine Apolipoprotein A-I Thyroid Molecular biology Endocrinology medicine.anatomical_structure Gene Expression Regulation Protein Biosynthesis lipids (amino acids peptides and proteins) Apolipoprotein A-II Hormone |
| Zdroj: | European Journal of Biochemistry European Journal of Biochemistry, Wiley, 1995, 231 (1), pp.126-132. ⟨10.1111/j.1432-1033.1995.tb20678.x⟩ European Journal of Biochemistry, Wiley, 1995, 231 (1), pp.126-132. ⟨10.1111/j.1432-1033.1995.0126f.x⟩ European Journal of Biochemistry, 1995, 231 (1), pp.126-132. ⟨10.1111/j.1432-1033.1995.tb20678.x⟩ European Journal of Biochemistry, 1995, 231 (1), pp.126-132. ⟨10.1111/j.1432-1033.1995.0126f.x⟩ |
| ISSN: | 0014-2956 1432-1327 |
| DOI: | 10.1111/j.1432-1033.1995.tb20678.x⟩ |
| Popis: | Hyperthyroidism is associated with elevated plasma levels of apolipoprotein AI (apo AI). We have examined the effects of 3,3′,-5-triiodothyronine on apo AI mRNA, transcription run-on activity, apo AI mRNA half-life, and the rate of protein synthesis in Hep G2 cells, to understand the molecular mechanism by which thyroid hormone regulates apo AI gene expression. Incubation with thyroid hormone increased the apo AI and apo AII mRNA concentrations twofold. Cycloheximide alone caused a significant increase in apo AI mRNA. Nuclear run-on assays indicate that thyroid hormone did not change the rate of the apo AI gene transcription at 6, 12 or 24 h, showing that thyroid hormone did not modulate apo AI gene transcription. Kinetic studies performed in the presence of actinomycin D showed that the half-life of apo AI mRNA was increased 2–3-fold by thyroid hormone over control cells. Thyroid hormone did not change the incorporation of [35S]methionine into immunoprecipitable apo AI. Pulse-chase experiments demonstrated that there was no change in the secretion and degradation rates of labeled apo AI in response to T3. This suggests that thyroid hormone does not affect the catabolism of apo AI (degradation or/and uptake) and that translation control strongly influences the regulation of apo AI gene expression. The stabilization of apo AI mRNA by thyroid hormone and its role in translation remain to be elucidated. |
| Databáze: | OpenAIRE |
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