The rapid identification of Clostridium perfringens as the possible aetiology of a diarrhoeal outbreak using PCR
| Autor: | Yih Yng Ng, Eng Shi Ong, M. B. Taylor, S. W. Chew, J. M. Fam, J. P. Loh, Eng Eong Ooi, Y. C. Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Diarrhea
Genotype Epidemiology Clostridium perfringens Enterotoxin medicine.disease_cause Polymerase Chain Reaction Microbiology law.invention Disease Outbreaks Enterotoxins Feces law medicine Humans Clostridiaceae Polymerase chain reaction Singapore biology Outbreak biology.organism_classification Original Papers Infectious Diseases Clostridium Infections medicine.symptom Food contaminant |
| Popis: | SUMMARYA gastroenteritis outbreak occurred in a military camp where a laboratory and epidemiological investigation was carried out. The early onset of symptoms indicated probable food contamination withClostridium perfringens. Stool samples collected from affected patients were tested within 4 h via real-time polymerase chain reaction (PCR) for the presence of theC. perfringens plcgene. Ten out of the 12 stool samples were positive. Confirmation of the molecular test results was carried out by enumeration ofC. perfringensin stool by culture and shown to be in excess of 106spores/g stool. The isolates obtained from culture were further analysed by PCR for the presence of the chromosomal enterotoxin (cpe) gene. Based on the clinical symptoms, epidemiological and laboratory investigations,C. perfringenswas implicated as the aetiological agent. The ability to conduct real-time PCR analysis greatly shortens the time to diagnosis and allows for preventive and control measures to be effected quickly. |
| Databáze: | OpenAIRE |
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