Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
| Autor: | Yoon-Kyoung Cho, Won-Seok Lee, Vijaya Sunkara, Ja-Ryoung Han, Yubin Kim, Yang-Seok Park |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
General Chemical Engineering Microfluidics Bioengineering Enzyme-Linked Immunosorbent Assay 02 engineering and technology 010402 general chemistry 01 natural sciences Protein detection General Biochemistry Genetics and Molecular Biology Lab-On-A-Chip Devices medicine Whole blood medicine.diagnostic_test General Immunology and Microbiology Small volume General Neuroscience Troponin I Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Blood proteins 0104 chemical sciences C-Reactive Protein Fully automated Immunoassay 0210 nano-technology Sensitivity (electronics) Biomarkers Biomedical engineering |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/54143-v |
| Popis: | Enzyme-linked immunosorbent assay (ELISA) is a promising method to detect small amount of proteins in biological samples. The devices providing a platform for reduced sample volume and assay time as well as full automation are required for potential use in point-of-care-diagnostics. Recently, we have demonstrated ultrasensitive detection of serum proteins, C-reactive protein (CRP) and cardiac troponin I (cTnI), utilizing a lab-on-a-disc composed of TiO2 nanofibrous (NF) mats. It showed a large dynamic range with femto molar (fM) detection sensitivity, from a small volume of whole blood in 30 min. The device consists of several components for blood separation, metering, mixing, and washing that are automated for improved sensitivity from low sample volumes. Here, in the video demonstration, we show the experimental protocols and know-how for the fabrication of NFs as well as the disc, their integration and the operation in the following order: processes for preparing TiO2 NF mat; transfer-printing of TiO2 NF mat onto the disc; surface modification for immune-reactions, disc assembly and operation; on-disc detection and representative results for immunoassay. Use of this device enables multiplexed analysis with minimal consumption of samples and reagents. Given the advantages, the device should find use in a wide variety of applications, and prove beneficial in facilitating the analysis of low abundant proteins. |
| Databáze: | OpenAIRE |
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