Addition of Purified Prostate Specific Antigen to Serum from Female Subjects
| Autor: | Kazuto Komatsu, Zuxiong Chen, Anthony Prestigiacomo, Thomas A. Stamey |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Serine Proteinase Inhibitors alpha 1-Antichymotrypsin Urology urologic and male genital diseases Alpha 1-antichymotrypsin alpha-2-Macroglobulin Western blot In vivo Prostate Internal medicine Medicine Humans alpha-Macroglobulins Immunoassay medicine.diagnostic_test biology business.industry Prostate-Specific Antigen Molecular biology Prostate-specific antigen Endocrinology medicine.anatomical_structure biology.protein Female Semenogelase business |
| Zdroj: | The Journal of Urology. :1357-1363 |
| ISSN: | 0022-5347 |
| DOI: | 10.1097/00005392-199610000-00028 |
| Popis: | Two forms of prostate specific antigen (PSA), 1 complexed to alpha 1-antichymotrypsin and the other a free PSA, are recognized by current commercially available immunoassays. A third form of PSA complexed to alpha 2-macroglobulin also is present in the serum. To study these 3 different molecular forms of PSA in vivo, we simulated leakage of PSA from the prostate into the serum in vitro.Purified seminal fluid PSA was incubated with fresh sera from female subjects at different concentrations. Following gel filtration chromatography, the 3 forms of PSA were studied by immunoassays and Western blot analysis.Using a commercial immunoassay, 60% of immunoreactivity of seminal fluid PSA was lost after incubation with sera from female subjects. Western blot analysis showed that most of this loss in PSA signal was caused by complexation to alpha 2-macroglobulin. Minimal, if any, complexation to alpha 1-antichymotrypsin occurred even when excess alpha 1-antichymotrypsin was added to the serum.Our studies demonstrated that alpha 2-macroglobulin is a much stronger inhibitor to PSA than alpha 1-antichymotrypsin. Further studies of these complexes may be important. They clearly explain why spiking PSA into sera from female subjects to be used as quality controls for PSA assays leads only to the free form of enzymatically inactive PSA in the serum, and not to the dominant form of complexed PSA and alpha 1-antichymotrypsin present in human serum. |
| Databáze: | OpenAIRE |
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