Conversion of positive to negative chemotaxis by Ca2+ ionophore treatment in plasmodium of Physarum polycephalum
| Autor: | Yasuo Mito, Hajime Satoh, Kenzo Kurihara, Yonosuke Kobatake, Tetsuo Ueda |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Lasalocid
Cell Membrane Permeability Contraction (grammar) Sucrose Movement Biophysics Aequorin Ionophore Physarum polycephalum Models Biological Biochemistry Physarum chemistry.chemical_compound Molecular Biology Calcimycin Alanine biology Negative chemotaxis Chemotaxis fungi biology.organism_classification Anti-Bacterial Agents chemistry biology.protein Calcium |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 633:436-443 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(80)90201-9 |
| Popis: | The plasmodium of Physarum polycephalum was treated with Ca 2+ ionophores A23187 or X-537A, and the effects on the chemotactic and contractile activities were studied by applying a double-chamber method and an optical method. 1. (1) At low Ca 2+ concentration, attractants (glucose, L -alanine, KH 2 PO 4 ) remained as attractants and induced a relaxation in the plasmodial strand. Increase in the external Ca 2+ level caused a contraction of the plasmodium with the same stimuli, and hence converted the attractants to repellents. 2. (2) This phenomenon was specific to Ca 2+ ; Mg 2+ , Sr 3+ or Ba 2+ showed no appreciable effects. 3. (3) Repellents (sucrose, KCl, Quinine) induced a contraction, which was not altered by the Ca 2+ ionophore treatment. 4. (4) Efflux of Ca 2+ , which was measured by chemiluminescence of aequorin, increased on glucose reception in the presence of ionophore. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|