Key role for efflux in the preservative susceptibility and adaptive resistance of Burkholderia cepacia complex bacteria
| Autor: | Adam Baldwin, Laura Rushton, Christopher G. Dowson, Andrea Sass, Denise Donoghue, Eshwar Mahenthiralingam |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Preservative
Parabens Microbial Sensitivity Tests Phenoxyethanol Microbiology chemistry.chemical_compound QH301 Bacterial Proteins Mechanisms of Resistance Drug Resistance Multiple Bacterial Sodium Benzoate Humans Pharmacology (medical) Occupational Health Pharmacology Base Sequence biology Benzethonium chloride Benzisothiazolinone Burkholderia cepacia complex Hydantoins Preservatives Pharmaceutical Burkholderia Infections Sequence Analysis DNA biology.organism_classification Bacterial Typing Techniques QR Thiazoles Infectious Diseases chemistry Biofilms Benzethonium Sodium benzoate Ethylene Glycols Efflux DNA Topoisomerases Bacteria Fluoroquinolones Multilocus Sequence Typing |
| ISSN: | 0066-4804 |
| Popis: | Bacteria from the Burkholderia cepacia complex (Bcc) are encountered as industrial contaminants, and little is known about the species involved or their mechanisms of preservative resistance. Multilocus sequence typing (MLST) revealed that multiple Bcc species may cause contamination, with B. lata ( n = 17) and B. cenocepacia ( n = 11) dominant within the collection examined. At the strain level, 11 of the 31 industrial sequence types identified had also been recovered from either natural environments or clinical infections. Minimal inhibitory (MIC) and minimum bactericidal (MBC) preservative concentrations varied across 83 selected Bcc strains, with industrial strains demonstrating increased tolerance for dimethylol dimethyl hydantoin (DMDMH). Benzisothiazolinone (BIT), DMDMH, methylisothiazolinone (MIT), a blend of 3:1 methylisothiazolinone-chloromethylisothiazolinone (M-CMIT), methyl paraben (MP), and phenoxyethanol (PH), were all effective anti-Bcc preservatives; benzethonium chloride (BC) and sodium benzoate (SB) were least effective. Since B. lata was the dominant industrial Bcc species, the type strain, 383 T (LMG 22485 T ), was used to study preservative tolerance. Strain 383 developed stable preservative tolerance for M-CMIT, MIT, BIT, and BC, which resulted in preservative cross-resistance and altered antibiotic susceptibility, motility, and biofilm formation. Transcriptomic analysis of the B. lata 383 M-CMIT-adapted strain demonstrated that efflux played a key role in its M-CMIT tolerance and elevated fluoroquinolone resistance. The role of efflux was corroborated using the inhibitor l -Phe-Arg-β-napthylamide, which reduced the MICs of M-CMIT and ciprofloxacin. In summary, intrinsic preservative tolerance and stable adaptive changes, such as enhanced efflux, play a role in the ability of Bcc bacteria to cause industrial contamination. |
| Databáze: | OpenAIRE |
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