Expression of the Matrix Metalloproteases 2, 14, 24, and 25 and Tissue Inhibitor 3 as Potential Molecular Markers in Advanced Human Gastric Cancer

Autor: Sol de la Peña, Mariana Ochoa-Lara, José María Remes-Troche, Kenneth León-Córdoba, Clara Luz Sampieri
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Disease Markers, Vol 2014 (2014)
Disease Markers
ISSN: 0278-0240
DOI: 10.1155/2014/285906
Popis: Background. During progression of gastric cancer (GC), degradation of the extracellular matrix is mediated by the matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs): changes in the expression of these have been related to unfavorable prognosis in GC.Objective. To analyze the expression of certain MMPs and TIMPs in chronic superficial gastritis (SG) and GC.Methods. The expression of MMPs and TIMPs was determined using qRT-PCR; the expression was classified, using threshold cycle (CT) values, as very high (CT≤25), high (CT=26–30), moderate (CT=31–35), low (CT=36–39), or not detected (CT=40). Strength of association was estimated between the proteins, which were detected by Western blot, and the risk of developing GC.Results. We found a high expression ofMMP1,MMP2,MMP14,TIMP1,andTIMP3; moderate one ofMMP9andMMP25,and low one ofMMP13andMMP24in both tissues. In absolute mRNA levels, significant differences were found in expression ofMMP2,MMP24,andMMP25, which are overexpressed in GC compared with SG. The presence of the proteins MMP-14 and TIMP-3 was associated with the risk of developing GC.Conclusions. We consider thatMMP2,MMP24,andMMP25and the proteins MMP-14 and TIMP-3 could be candidates for prognostic molecular markers in GC.
Databáze: OpenAIRE
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