Genome editing by CRISPR-Cas9 technology in Petunia hybrida
| Autor: | Mathilde Chopy, S. Rodrigues Bento, Michiel Vandenbussche, Pierre Morel |
|---|---|
| Přispěvatelé: | Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences biology [SDV]Life Sciences [q-bio] Computational biology Horticulture biology.organism_classification 01 natural sciences Null allele Petunia Reverse genetics targeted mutation 03 medical and health sciences reverse genetics Targeted Mutation Genome editing flower development CRISPR genome editing petunia Guide RNA CRISPR-Cas9 Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Acta Horticulturae Acta Horticulturae, 2020, pp.209-218. ⟨10.17660/ActaHortic.2020.1283.28⟩ Acta Horticulturae, 2020, 1283, pp.209-218. ⟨10.17660/ActaHortic.2020.1283.28⟩ |
| ISSN: | 0567-7572 |
| Popis: | International audience; CRISPR-Cas9 technology has been shown to be a very powerful tool to generate targeted mutations in a wide range of species. To evaluate its applicability for standard gene functional analysis in Petunia, we performed a case study aimed at obtaining loss-of-function alleles for six different Petunia genes. Using single guide RNAs (sgRNA), we obtained between 1 to 3 putative null alleles for 5 out of the six genes. Here we describe in detail the obtained results and the protocol used in this study, and discuss some of the technical problems encountered. Although the efficiency of the protocol should be further increased, these results demonstrate that CRISPR-Cas9 technology represents a very useful addition to the available molecular toolkit in petunia. |
| Databáze: | OpenAIRE |
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