| Popis: |
The purpose of the study was to determine protein profile from catfish (Pangasius hypophthalmus) andbaung fish (Hemibagrus nemurus) muscle from South Kalimantan, Indonesia. The protein was extracted with Tris-EDTA buffer, followed precipitation with 20-30%, 30-40%, 40-50%, 50-60%, 60-70%, and 70-80%of ammonium sulfate. The protein concentrations were analyzing using Bradford reagent, then the molecularweight of protein was performed on SDS-PAGE. The highest concentration of protein from catfish (4.24±0.03 µg/µl) and baung fish (4.25±0.03µg/µl) were observed in the 60-70% and 70-80% saturation of ammonium sulfate. Twenty of protein bands from catfish were shown in 10% resolving gel and have molecular weight 24 to 146 kDa, respectively. Fourteen of protein bands from baung fish have molecular weight 25 to 123 kDa, respectively. The bands were characterized as C-protein, gelsolin, actin, tropomyosin, troponin and myosin light chain. These results allow a better understanding of the protein profile of catfish and baung fish and for the basisof the development of an immunoassay for the detection of fish muscle. |
