| Popis: |
We have previously identified a novel non-selective membrane conductance (gsubUS/sub) opened by focused ultrasound (FUS) in crayfish motor axons. In the work described here, we studied gsubUS/subproperties further by comparing FUS-evoked depolarization (FUSD) in control and hypotonic saline with 75% of control osmolarity. The FUS was a train of 20 FUS bursts (2.1 MHz and 50 µs per burst) delivered at 1 kHz. The amplitude, onset latency, frequency of occurrence and duration of FUSD were compared in a 15-min time window before and after switching to hypotonic saline. Significant increases were observed for amplitude (plt; 0.001) and frequency of occurrence (plt; 0.01) while the onset latency exhibited a significant decrease (plt; 0.001). FUSD duration did not significantly differ. These results support predictions based on our hypothesis that gsubUS/subis mediated by opening of nanopores in the lipid bilayer and that stretching of axonal membrane caused by swelling at low osmolarity should increase the probability of nanopore formation under FUS. The FUSD parameters, in addition, exhibited time-dependent trends when the window of observation was expanded to 45 min in each saline. The statistical significance of amplitude and duration differed between 15- and 45-min time windows, indicating the presence of adaptive responses of axonal membrane to osmotic manipulation. |
