Acute hepatic steatosis in mice by blocking beta-oxidation does not reduce insulin sensitivity of very-low-density lipoprotein production
| Autor: | Jildou Hoekstra, Terry G J Derks, Julius F. W. Baller, D. Margriet Ouwens, Folkert Kuipers, Rick Havinga, Louis M. Havekes, Aldo Grefhorst, Johannes A. Romijn |
|---|---|
| Přispěvatelé: | Other departments, Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
Very low-density lipoprotein Physiology medicine.medical_treatment Cholesterol VLDL chemistry.chemical_compound Mice Phosphatidylinositol 3-Kinases tetradecylglycidic acid Insulin Beta oxidation triglycerides Fatty liver Fatty Acids Gastroenterology Acute Disease VLDL PRODUCTION SHORT-TERM SECRETION carnitine palmitoyl transferase-1 FATTY-ACIDS Oxidation-Reduction medicine.medical_specialty APOLIPOPROTEIN-B Biology METABOLISM Protein Serine-Threonine Kinases Insulin resistance Physiology (medical) Internal medicine Proto-Oncogene Proteins medicine Animals Hypoglycemic Agents Liver X receptor DE-NOVO LIPOGENESIS Hepatology RAT HEPATOCYTES Cholesterol medicine.disease Acute hepatic steatosis Fatty Liver Mice Inbred C57BL Endocrinology chemistry Epoxy Compounds 2-TETRADECYLGLYCIDIC ACID Insulin Resistance LIVER-X-RECEPTOR Proto-Oncogene Proteins c-akt |
| Zdroj: | American journal of physiology. Gastrointestinal and liver physiology, 289(3), G592-G598. American Physiological Society American Journal of Physiology-Gastrointestinal and Liver Physiology, 289(3), G592-G598. AMER PHYSIOLOGICAL SOC |
| ISSN: | 0193-1857 |
| DOI: | 10.1152/ajpgi.00063.2005 |
| Popis: | Accumulation of triglycerides (TG) in the liver is generally associated with hepatic insulin resistance. We questioned whether acute hepatic steatosis induced by pharmacological blockade of beta-oxidation affects hepatic insulin sensitivity, i.e., insulin-mediated suppression of VLDL production and insulin-induced activation of phosphatidylinositol 3-kinase (PI3-kinase) and PKB. Tetradecylglycidic acid (TDGA), an inhibitor of carnitine palmitoyl transferase-1 (CPT1), was used for this purpose. Male C57BL/6J mice received 30 mg/kg TDGA or its solvent intraperitoneally and were subsequently fasted for 12 h. CPT1 inhibition resulted in severe microvesicular hepatic steatosis (19.9 +/- 8.3 vs. 112.4 +/- 25.2 nmol TG/mg liver, control vs. treated, P |
| Databáze: | OpenAIRE |
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