Evaluation of novel protease enzymes on growth performance and apparent ileal digestibility of amino acids in poultry: enzyme screening
| Autor: | David R. Ledoux, K. Juntunen, Carrie L Walk, M. Paloheimo, Vasil Pirgozliev |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Turkeys Proteases Aspartic Acid Proteases medicine.medical_treatment Feed conversion ratio Eating Random Allocation 03 medical and health sciences Animal science Nutrient Ileum medicine Animals Amino Acids chemistry.chemical_classification Protease Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Animal Feed 040201 dairy & animal science Diet Amino acid 030104 developmental biology Enzyme Dietary Supplements Xylanase Animal Nutritional Physiological Phenomena Digestion Animal Science and Zoology Phytase Serine Proteases Chickens |
| Zdroj: | Poultry Science. 97:2123-2138 |
| ISSN: | 0032-5791 |
| DOI: | 10.3382/ps/pey080 |
| Popis: | Three experiments were conducted to evaluate eight neutral and six acid proteases on growth performance and apparent ileal amino acid digestibility (AID) of poults (Experiment 1) or chicks (Experiments 2 and 3). Two basal diets were formulated: a nutrient adequate positive control (PC), which met or exceeded the nutrient requirements for poults (Experiment 1) or chicks (Experiments 2 and 3) and a negative control (NC) formulated to achieve 85% (Experiments 1 and 2) or 80% (Experiments 3) of the requirement for protein and amino acids. Phytase was included in all diets to provide 500 phytase units (FTU)/kg and xylanase was included in all diets to provide 10,000 (Experiments 1 and 2) or 16,000 (Experiments 3) xylanase units (BXU)/kg. Proteases were supplemented in the NC diet at an equivalent amount of enzyme protein to create 16 experimental diets. There were five birds/pen and 10 replicate pens per treatment in each experiment. In experiment 1, birds fed the PC diet gained more (P < 0.05) than birds fed the NC. There were no differences in growth performance in birds fed the PC or NC in experiments 2 or 3. In all three experiments, birds fed the NC supplemented with neutral protease 1 had reduced (P < 0.05) feed intake (FI) or body weight gain (BWG) and increased (P < 0.05) feed conversion ratio (FCR) compared with birds fed the NC. Birds fed the NC diet supplemented with neutral protease 3, 7 (Experiment 1), or acid protease 4 (Experiment 3) had increased (P < 0.05) FCR and birds fed neutral protease 6 (Experiment 2) had reduced (P < 0.05) BWG compared with birds fed the NC. Apparent ileal amino acid digestibility was improved (P < 0.05) with protease supplementation to the NC diets (Experiment 1 or 3), but this was dependent on the protease and the amino acid. In conclusion, novel protease supplementation improved AID of amino acids but this was not reflected in improvements in growth performance of poults or chicks. |
| Databáze: | OpenAIRE |
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