Icosahedral gold nanoparticles decorated with hexon protein: a surrogate for adenovirus serotype 5

Autor: Beatriz Fresco-Cala, Ángela I. López-Lorente, Alex D. Batista, Mehmet Dinc, Joachim Bansmann, R. Jürgen Behm, Soledad Cárdenas, Boris Mizaikoff
Rok vydání: 2022
Předmět:
Zdroj: Analytical and Bioanalytical Chemistry. 415:2081-2090
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-022-04368-x
Popis: The development of synthetic particles that emulate real viruses in size, shape, and chemical composition is vital to the development of imprinted polymer-based sorbent materials (molecularly imprinted polymers, MIPs). In this study, we address surrogates for adenovirus type 5 (Adv 5) via the synthesis and subsequent modification of icosahedral gold nanoparticles (iAuNPs) decorated with the most abundant protein of the Adv 5 (i.e., hexon protein) at the surface. CTAB-capped iAuNPs with dimensions in the range of 40-90 nm were synthesized, and then CTAB was replaced by a variety of polyethylene glycols (PEGs) in order to introduce suitable functionalities serving as anchoring points for the attachment of the hexon protein. The latter was achieved by non-covalent linking of the protein to the iAuNP surface using a PEG without reactive termination (i.e., methoxy PEG thiol, mPEG-SH, Mn=800). Alternatively, covalent anchoring points were generated by modifying the iAuNPs with a bifunctional PEG (i.e., thiol PEG amine, SH-PEG-NH
Databáze: OpenAIRE
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