Allele and genotype frequencies of CYP2B6 and CYP3A5 in the Japanese population
| Autor: | Naomi Endo, Masaki Matsuura, Yasuyuki Agatsuma, Tomoko Inoue, Kaori Narahara, Yoh Takekuma, Masahiro Hiratsuka, Yukinaga Kishikawa, Michinao Mizugaki, Samar Ismail Hamdy |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Genotype Biology Polymerase Chain Reaction Cytochrome P-450 Enzyme System Gene Frequency Japan Cytochrome P-450 CYP3A Humans Pharmacology (medical) Allele Allele frequency Genotyping Pharmacology Genetics Oxidoreductases N-Demethylating General Medicine Genotype frequency Minor allele frequency Cytochrome P-450 CYP2B6 Female Aryl Hydrocarbon Hydroxylases Restriction fragment length polymorphism Pharmacogenetics Polymorphism Restriction Fragment Length |
| Zdroj: | European journal of clinical pharmacology. 58(6) |
| ISSN: | 0031-6970 |
| Popis: | Objective. The goal of this study was to determine the frequencies of allelic variants of CYP2B6 and CYP3A5 in the Japanese population. Methods. Genotyping of CYP2B6(*2, *3, *4, *5, *6, and *7) and CYP3A5 (*2, *3, *4, *5, and *6) was carried out in 265 unrelated Japanese subjects by polymerase chain reaction (PCR), restriction fragment length polymorphism and allele-specific, real-time PCR assays. Results. Allele frequencies for CYP2B6*2, *3, *4, *5, *6, and *7 in 256 Japanese subjects were 0.047, 0, 0.093, 0.011, 0.164, and 0, respectively. Ethnic variation in allele frequencies relative to that in Caucasian subjects was observed for CYP2B6*4 (0.093 vs 0.040), *5 (0.011 vs 0.109), *6 (0.164 vs 0.256), and *7 (0 vs 0.030). Allele frequencies for CYP3A5*2, *3, *4, *5, and *6 in 265 Japanese subjects were 0, 0.740, 0, 0.004, and 0, respectively. The frequency of the CYP3A5*1 allele is 2.8 times higher in Japanese than in Caucasians. Conclusions. Our results contribute to a better understanding of the molecular basis of ethnic differences in drug response, which may help to improve individualization of drug therapy and offer a preliminary basis for more rational use of drugs that are substrates for CYP2B6 and CYP3A5 in the Japanese population. |
| Databáze: | OpenAIRE |
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