Hyperoxia-induced ciliary loss and oxidative damage in an in vitro bovine model: The protective role of antioxidant vitamins E and C
| Autor: | Hanady Salim Al-Shmgani, J. Robert Sneyd, Roy Moate, Peter Macnaughton, A. John Moody |
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| Rok vydání: | 2012 |
| Předmět: |
Antioxidant
medicine.medical_treatment Biophysics Bronchi Ascorbic Acid Respiratory Mucosa Hyperoxia Biology Protein oxidation medicine.disease_cause Biochemistry Antioxidants Andrology Lipid peroxidation chemistry.chemical_compound Intensive care Lactate dehydrogenase medicine TBARS Animals Vitamin E Cilia Molecular Biology Cells Cultured L-Lactate Dehydrogenase Cell Biology Oxidative Stress chemistry Cytoprotection Cattle Lipid Peroxidation medicine.symptom Oxidative stress DNA Damage |
| Zdroj: | Biochemical and Biophysical Research Communications. 429:191-196 |
| ISSN: | 0006-291X |
| Popis: | Although elevated oxygen fraction is used in intensive care units around the world, pathological changes in pulmonary tissue have been shown to occur with prolonged exposure to hyperoxia. In this work a bovine bronchus culture model has been successfully used to evaluate the effects of hyperoxia on ciliated epithelium in vitro. Samples were cultured using an air interface method and exposed to normoxia, 21% O(2) or hyperoxia, 95% O(2). Cilial coverage was assessed using scanning electron microscopy (SEM). Tissue damage (lactate dehydrogenase, LDH, in the medium), lipid peroxidation (thiobarbituric acid reactive substances, TBARS), DNA damage (comet assay), protein oxidation (OxyBlot kit) and antioxidant status (total glutathione) were used to assess whether the hyperoxia caused significant oxidative stress. Hyperoxia caused a time-dependent decline (t(½)=3.4d compared to 37.1d under normoxia) in cilial coverage (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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