Lymphocytes from patients with early stage of B-cell chronic lymphocytic leukaemia and long survival synthesize decorin
| Autor: | Luana Calabrò, Alberto Calatroni, Angela D'Ascola, Salvatore Campo, Eugenia Quartarone, Angela Avenoso, Giuseppe M. Campo, Caterina Musolino, Giacomo Bellomo |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Chronic lymphocytic leukaemia Decorin Blotting Western Gene Expression Biology Biochemistry NO Western blot Transcription (biology) hemic and lymphatic diseases Biglycan Gene expression medicine Humans Lymphocytes RNA Messenger Aged Neoplasm Staging Aged 80 and over Extracellular Matrix Proteins Messenger RNA medicine.diagnostic_test Anti-oncogen Proteoglycans General Medicine Middle Aged Leukemia Lymphocytic Chronic B-Cell carbohydrates (lipids) Immunology Cancer research Female Intracellular Transforming growth factor |
| Zdroj: | Biochimie. 88:1933-1939 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2006.07.010 |
| Popis: | mRNA/cDNA gene expression of both small leucine-rich proteoglycans decorin and biglycan was evaluated by PCR real time in lymphocytes collected from patients with chronic lymphocytic leukaemia (CLL) at different stages of disease and from healthy controls. Lymphocytes obtained from healthy controls showed no or very low levels of mRNA expression of both decorin and biglycan. Biglycan expression was very low in CLL patients, values being close to those of controls. On the contrary, decorin mRNA was clearly expressed in patients with early B-cell CLL, while a low expression was found in advanced clinical stages. Furthermore, a significant higher decorin expression was found in patients with non-progressive CLL type in comparison with patients with aggressive type of the disease. Decorin expression resulted especially high in the low-progressive low-risk patients. The synthesis of decorin was also assessed by Western blot analysis. The peculiar occurrence of decorin in the non-aggressive type of CLL is consistent with its suggested anti-oncogenic role. Intracellular Bcl-2 level does not correlate with decorin mRNA transcription, suggesting that a Bcl-2 independent anti-cancer mechanism may occur. The measurement of galactosamine-containing proteoglycans concentration in plasma confirmed decorin expression results, with significant differences between CLL patients and controls. Significant changes were also seen between groups of patients of Rai stage 0 with recent diagnosis (less than 5 years, from analysis), (low amount of decorin) and less recent diagnosis (more than 5 years), (high amount of decorin). |
| Databáze: | OpenAIRE |
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