FURTHER STUDIES ON THE ANTIGENS OF ESCHERICHIA COLI O26:B6
| Autor: | W R Bailey, J E McDade |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Immunodiffusion
Hot Temperature Escherichia coli serotype Antigen-antibody reactions Chemistry Immunology Cell General Medicine medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Molecular biology Antigen-Antibody Reactions medicine.anatomical_structure Antigen Escherichia coli Genetics medicine Antigens Molecular Biology |
| Zdroj: | Canadian Journal of Microbiology. 12:249-254 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m66-034 |
| Popis: | The effect of heat on the O and B antigens of Escherichia coli serotype O26:B6 was studied. Aliquots of cell suspensions containing 1.5 × 1010 cells/ml were heated at 55, 65, 75, 85, and 100 °C, respectively, for 1 hour. The heated cells were centrifuged and the precipitinogen titer determined using O and OB antisera. The supernatants from cell suspensions heated at the higher temperatures contained proportionately more of the soluble antigen than those heated at the lower temperatures.Gel diffusion tests of the supernatants showed that both O and B antigens were released when cells were heated. Mild acid hydrolysis of a supernatant increased the B reactivity of that supernatant and slightly enhanced the O reaction. It was concluded from this study that the O and B antigens exist as a molecular complex; heating releases the B component from the complex and causes the increased agglutination with O antiserum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|