Distinct metabolic effects of resveratrol on lipogenesis markers in mice adipose tissue treated with high-polyunsaturated fat and high-protein diets
| Autor: | Alfredo Maurício Batista de Paula, Sérgio Henrique Sousa Santos, Simone Moreira de Macedo, Jamille Fernandes Lula, Keila Lopes Mendes, João Marcus Oliveira Andrade, Alanna Fernandes Paraíso, Lucinéia de Pinho, André Luiz Sena Guimarães, John David Feltenberger |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Adipose tissue 030204 cardiovascular system & hematology Biology Resveratrol Fitonutrientes General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences chemistry.chemical_compound Polyunsaturated fat Síndrome metabólica 0302 clinical medicine Dietary Fats Unsaturated Internal medicine Adipocyte Stilbenes medicine Animals General Pharmacology Toxicology and Pharmaceutics Adipogenesis Triglyceride Cholesterol Lipogenesis food and beverages General Medicine medicine.disease 030104 developmental biology Endocrinology Adipose Tissue chemistry Body Composition Fatty Acids Unsaturated Female lipids (amino acids peptides and proteins) Dietary Proteins Metabolic syndrome Colesterol |
| Zdroj: | Repositório Institucional da UFMG Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 0024-3205 |
| Popis: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Objective: a healthy diet is essential for the prevention of metabolic syndrome. The present study evaluated the effect of resveratrol associated with high-polyunsaturated fat and high-protein diets on expression of adipogenic and lipogenic genes. Research methods & procedures: FVB/N mice were divided into 6 groups (n = 7 each) and fed with experimental diets for 60 days: standard (ST), high-fat diet (HFD), and high-protein diet (HPD), with and without resveratrol (RSV) (4 g/kg diet). The body weight, food intake, energy intake (kcal), and blood parameters (HDL-C, total cholesterol, glucose, and triglyceride levels) were assessed. Real-time PCR was performed to analyze the expression of adipogenesis and lipogenesis markers: PPARγ, SREBP-1c, ACC and FAS in samples from perigonadal adipose tissue. Results: in the HPD + RSV group, resveratrol decreased body weight, body adiposity, adipose tissue weight, adipocyte area, total cholesterol, ACC and FAS expression, and increased HDL-cholesterol in comparison to HPD. In the HPD group there was a decrease in adipocyte area, as well as PPARγ, SREBP-1c and ACC expression in comparison to ST. While in HFD + RSV, resveratrol decreased levels of total cholesterol in comparison to HFD. In the HFD group there was decrease in body weight, and PPARγ, SREBP-1c and ACC expression in comparison to ST. Conclusions: the obtained results show that resveratrol decreases lipogenesis markers and metabolic parameters in the setting of a high-protein diet. Moreover, resveratrol decreased total cholesterol in both diets. These results point to the increased potential of resveratrol use in prevention of metabolic syndrome, acting on different dietary compositions. |
| Databáze: | OpenAIRE |
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