Triplaris gardneriana seeds extract exhibits in vitro anti-inflammatory properties in human neutrophils after oxidative treatment
| Autor: | Thiago Silva de Almeida, Pablo A. Nogara, João Rocha, José Joaquim Lopes Neto, Talita Magalhães Rocha, Karise Fernanda Nogara, Luiz Carlos Pereira Almeida Filho, Luzia Kalyne Almeida Moreira Leal, Ana Fontenele Urano Carvalho |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Neutrophils medicine.drug_class medicine.medical_treatment Anti-Inflammatory Agents Anti-inflammatory Docking 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Discovery medicine Humans Gallic acid 030304 developmental biology Inflammation Pharmacology chemistry.chemical_classification 0303 health sciences Reactive oxygen species Myeloperoxidase Dose-Response Relationship Drug biology Plant Extracts Neutrophil Catechin Free Radical Scavengers Hydrogen Peroxide Polygonaceae Quercitrin Molecular Docking Simulation Oxidative Stress chemistry Biochemistry 030220 oncology & carcinogenesis Seeds biology.protein Reactive Oxygen Species Quercetin Triplaris gardneriana Brazil |
| Zdroj: | Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Triplaris gardneriana Wedd. (Polygonaceae family) is a plant species from Brazilian semiarid region which is used in local traditional medicine for the treatment of inflammatory conditions such as hemorrhoids. Aim of the study In this study, the in vitro anti-inflammatory activity of different concentrations of ethanolic extract from T. gardneriana seeds (EETg) was performed in order to contribute to the knowledge about etnomedicinal use of this plant species. Materials and methods The anti-inflammatory properties were evaluated through different approaches, such as in vitro protein anti-denaturation test, scavenging of reactive oxygen species (ROS) and myeloperoxidase (MPO) inhibition in human neutrophils activated by phorbol-12-myristate-13-acetate (PMA). Besides that, molecular docking was performed to provide new insights about the interaction between the major phenolic components in the plant extract and MPO. Results EETg was characterized showing a total phenol content of 153.5 ± 6.3 μg gallic acid equivalent/mg extract, ability to remove hydrogen peroxide (H2O2) in a concentration-dependent manner and had a spectroscopic profile which suggests the presence of hydroxyl groups. EETg was able to prevent protein denaturation ranging from 40.17 to 75.09%. The extract, at 10 and 20 μg/mL, was able to modulate neutrophils pro-inflammatory functions, such as degranulation and burst respiratory. In both assays, the EETg had anti-inflammatory effect comparable to nonsteroidal anti-inflammatory drugs. Among the main phenolic compounds of EETg, quercitrin, quercetin and catechin showed the highest binding affinity in silico to MPO. Conclusion This study demonstrated, for the first time, that the anti-inflammatory effect of T. gardneriana seeds occurs due to its modulatory effect on human neutrophil degranulation and free-radical scavenging activity. |
| Databáze: | OpenAIRE |
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