Novel bacteriocins produced by Lactobacillus fermentum strains with bacteriostatic effects in milk against selected indicator microorganisms
| Autor: | Miguel Ángel Rendon-Rosales, M. C. Estrada-Montoya, María J. Torres-Llanez, Ricardo Reyes-Díaz, Belinda Vallejo-Cordoba, Lilia M. Beltrán-Barrientos, Adrián Hernández-Mendoza, Aarón F. González-Córdova, Priscilia Y. Heredia-Castro |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Limosilactobacillus fermentum
Listeria Lactobacillus fermentum Fractionation medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound Bacteriocins Bacteriocin Genetics medicine Animals Food science Agar diffusion test Escherichia coli Nisin 030304 developmental biology 0303 health sciences biology 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences biology.organism_classification Antimicrobial 040201 dairy & animal science Anti-Bacterial Agents Lactobacillus Milk chemistry Food Microbiology Animal Science and Zoology Food Science |
| Zdroj: | Journal of Dairy Science. 104:4033-4043 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2020-19531 |
| Popis: | The aim of this work was to isolate and characterize bacteriocins produced by 2 Lactobacillus fermentum strains isolated from artisanal Mexican Cocido cheese. Fractions (F ≤3 kDa) obtained from cell-free supernatants of Lb. fermentum strains J23 and J32 were further fractionated by reversed-phase HPLC on a C18 column. Antimicrobial activities of F ≤3 kDa and bacteriocin-containing fractions (BCF), obtained from fractionation of F ≤3 kDa against 4 indicator microorganisms, were determined by the disk diffusion method and growth inhibition in milk. Subsequently, isolated BCF were analyzed by reversed-phase HPLC tandem mass spectrometry. Results showed that BCF presented antimicrobial activity against the 4 indicator microorganisms tested. For J23, one of the fractions (F3) presented the highest activity against Escherichia coli and was also inhibitory against Staphylococcus aureus, Listeria innocua, Salmonella Typhimurium, and Salmonella Choleraesuis. Similarly, fractions F3 and F4 produced by J32 presented antimicrobial activity against all indicator microorganisms. Furthermore, generation time and growth rate showed that F3 from J23 presented significantly higher antimicrobial activity against the 4 indicator microorganisms (2 gram-positive and 2 gram-negative) when inoculated in milk compared with F3 from J32. Interestingly, this fraction presented a broader antimicrobial spectrum in milk than nisin (control). Reversed-phase HPLC tandem mass spectrometry analysis revealed the presence of several peptides in BCF; however, F3 from J23 that was the most active fraction of all presented only 1 bacteriocin. The chemical characterization of this bacteriocin suggested that it was a novel peptide with 10 hydrophobic AA residues in its sequence and a molecular weight of 2,056 Da. This bacteriocin and its producing strain, J23, may find application as a biopreservative against these indicator microorganisms in dairy products. |
| Databáze: | OpenAIRE |
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