JAK/STAT Dysregulation With SOCS1 Overexpression in Acquired Cholesteatoma-Adjacent Mucosa
| Autor: | Elina Mäki-Torkko, Ellen Tideholm, Krzysztof Piersiala, Johanna Westerberg, Cecilia Drakskog, Susanna Kumlien Georén, Lars-Olaf Cardell |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Thymic stromal lymphopoietin
medicine.medical_treatment Transducers Mastoidectomy 03 medical and health sciences Suppressor of Cytokine Signaling 1 Protein 0302 clinical medicine otorhinolaryngologic diseases medicine Humans Cholesteatoma 030223 otorhinolaryngology Janus Kinases Sweden Mucous Membrane biology Suppressor of cytokine signaling 1 business.industry JAK-STAT signaling pathway Transforming growth factor beta medicine.disease Sensory Systems Otorhinolaryngology Case-Control Studies Cancer research biology.protein STAT protein Neurology (clinical) Neoplasm Recurrence Local Janus kinase business 030217 neurology & neurosurgery |
| Zdroj: | Otology & Neurotology. 42:e94-e100 |
| ISSN: | 1537-4505 1531-7129 |
| DOI: | 10.1097/mao.0000000000002850 |
| Popis: | Importance Surgery remains the gold standard in cholesteatoma treatment. However, the rate of recurrence is significant and the development of new nonsurgical treatment alternatives is warranted. One of the possible molecular pathways to target is the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway. Objective To investigate the JAK/STAT pathway in the middle ear mucosa in patients with acquired cholesteatoma compared with middle ear mucosa from healthy controls. Design Case-control study. Setting Linkoping University Hospital, Sweden, and Karolinska Institutet, Stockholm, Sweden. Sampling period: February 2011 to December 2016. Participants Middle ear mucosa from 26 patients with acquired cholesteatoma undergoing tympanoplasty and mastoidectomy, and 27 healthy controls undergoing translabyrinthine surgery for vestibular schwannoma or cochlear implantation was investigated. Main outcomes/measures The expression of Interleukin-7 receptor alpha, JAK1, JAK2, JAK3, STAT5A, STAT5B, and suppressor of cytokine signaling-1 (SOCS1) were quantified using quantitative polymerase chain reaction. In addition, expression level of cyclin D2, transforming growth factor beta 1, thymic stromal lymphopoietin, CD3, and CD19 was evaluated. Results In cholesteatoma-adjacent mucosa, SOCS1 was significantly upregulated (p= 0.0003) compared with healthy controls, whereas STAT5B was significantly downregulated (p = 0.0006). The expression of JAK1, JAK2, JAK3, and STAT5A did not differ significantly between groups. Conclusions and relevance To the best of our knowledge, this is the first article reporting dysregulation of the JAK/STAT pathway in cholesteatoma-adjacent mucosa. The main finding is that important players of the aforementioned pathway are significantly altered, namely SOCS1 is upregulated and STAT5B is downregulated compared with healthy controls. |
| Databáze: | OpenAIRE |
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