Proglucagon cDNAs from the Leopard Frog, Rana pipiens, encode two GLP-1-like peptides
| Autor: | Prashanth Sivarajah, David M. Irwin |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
DNA Complementary Molecular Sequence Data Molecular cloning Proglucagon Biochemistry Glucagon Evolution Molecular Exon Endocrinology Glucagon-Like Peptide 1 Glucagon-Like Peptide 2 Animals Humans Amino Acid Sequence RNA Messenger Intestinal Mucosa Protein Precursors Pancreas Molecular Biology Phylogeny DNA Primers Base Sequence Sequence Homology Amino Acid biology Rana pipiens digestive oral and skin physiology Alternative splicing Leopard frog Glucagon-like peptide-2 biology.organism_classification Glucagon-like peptide-1 Molecular biology Peptide Fragments Alternative Splicing Peptides hormones hormone substitutes and hormone antagonists |
| Zdroj: | Molecular and Cellular Endocrinology. 162:17-24 |
| ISSN: | 0303-7207 |
| Popis: | We have isolated and characterized proglucagon cDNAs from the intestine and pancreas of the leopard frog Rana pipiens. R. pipiens proglucagon encodes glucagon, glucagon-like peptides 2 (GLP-2), and two glucagon-like peptide 1 (GLP-1) like sequences. The pancreatic and intestinal cDNAs were of identical structure and sequence suggesting that, unlike many other non-mammalian vertebrates, there is little or no alternative splicing of the proglucagon mRNA in this species. A phylogenetic analysis of the GLP-1 encoding sequences implies that the exon encoding GLP-1 was triplicated early in frog evolution, more than 150 million years ago, before the divergence of modern frogs. |
| Databáze: | OpenAIRE |
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