Rapid identification ofAcremonium loliiandAcremonium coenophialumendophytes through arbitrarily primed PCR
Autor: | P. Cunningham, R. van Heeswijck, G. Latch, T. Leonforte, D. Liua, C. Langford, K. Reed, M. Panaccio |
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Rok vydání: | 1995 |
Předmět: |
Molecular Sequence Data
Polymerase Chain Reaction Microbiology Endophyte Acremonium coenophialum law.invention chemistry.chemical_compound law Lolium Genetics DNA Fungal Molecular Biology Polymerase chain reaction DNA Primers Base Sequence biology Acremonium Fungal genetics Fungi imperfecti biology.organism_classification Rapid identification chemistry DNA Probes DNA |
Zdroj: | FEMS Microbiology Letters. 133:95-98 |
ISSN: | 1574-6968 0378-1097 |
DOI: | 10.1111/j.1574-6968.1995.tb07867.x |
Popis: | Using a random decamer 5'-CCGAGGTGAC-3' in an arbitrarily primed PCR, similar band patterns were observed between Acremonium lolii and A. coenophialum DNA, which were somewhat different from those formed by other fungal DNA. Despite sharing bands of around 0.7, 0.9 and 2.1 kb, A. lolii can be distinguished from A. coenophialum by the presence of an additional band at around 0.5 kb in the arbitrarily primed PCR. |
Databáze: | OpenAIRE |
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