Mild portal endotoxaemia induces subacute hepatic inflammation and pancreatic β-cell dysfunction in rats
| Autor: | Ch H. Loh, J. Y‐H. Chan, P‐S. Hsieh, J‐F. Shyu, Y‐T. Chen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Necrosis medicine.medical_treatment Clinical Biochemistry Inflammation Biochemistry Hepatitis Pathogenesis Risk Factors Superoxides Insulin-Secreting Cells White blood cell Internal medicine Insulin Secretion TBARS Animals Insulin Medicine Rats Wistar business.industry Pancreatic islets General Medicine Endotoxemia Rats Portal System medicine.anatomical_structure Endocrinology medicine.symptom business Pancreas |
| Zdroj: | European Journal of Clinical Investigation. 38:640-648 |
| ISSN: | 1365-2362 0014-2972 |
| DOI: | 10.1111/j.1365-2362.2008.01991.x |
| Popis: | BACKGROUND Portal endotoxaemia has been speculated to be crucially involved in the pathogenesis of chronic hepatic inflammation, which is highly associated with the development of type 2 diabetes mellitus. This study tests whether portal endotoxaemia is a pathogenic link between chronic subacute hepatic inflammation and pancreatic beta-cell dysfunction. MATERIALS AND METHODS Rats were randomly assigned into two groups: rats with intraportal saline or low-dose lipopolysaccharide (LPS) infusion for 4 weeks. Pathological changes in the liver were evaluated via histological and biochemical examination. Pancreatic insulin secretion was evaluated by in vivo hyperglycaemic clamp study. RESULTS White blood cell count was significantly increased after intraportal LPS infusion for 4 weeks. Plasma amylase and chemoluminescence counts indicating superoxide levels were significantly increased after LPS treatments for 2 and 4 weeks. Intraportal low-dose LPS infusion significantly increased tumour necrosis factor-alpha and interleukin-6 contents in liver and pancreas. Circulating C-reactive protein, thiobarbituric acid reactive substances (TBARS) and endotoxin levels were not different among groups. The first- and second-phase insulin secretions in hyperglycaemic clamp were significantly decreased in LPS-treated rats. The histopathological scores, de novo production of reactive oxygen substrate and TBARS contents in the liver and pancreas were significantly increased in LPS-infused rats. Leucocyte infiltration was clearly visible in pancreatic islets of LPS-treated rats. CONCLUSIONS The present study demonstrated that mild portal endotoxaemia caused subacute hepatic inflammation and impaired pancreatic insulin secretion, implicating that portal endotoxaemia is a potential risk factor to link chronic subacute hepatic inflammation and pancreatic beta-cell dysfunction. |
| Databáze: | OpenAIRE |
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