Long noncoding RNAs C2dat1 enhances vascular smooth muscle cell proliferation and migration by targeting MiR-34a-5p
| Autor: | Gangchen Zhang, Wen-Hao Song, Yao Gong, Lin Zhang, Meichun Zhang, Hai-Rong Wang, Tu Pei, Deliang Chen, Zhili Jin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vascular smooth muscle Cell Myocytes Smooth Muscle Becaplermin Coronary Artery Disease Biochemistry Muscle Smooth Vascular 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Sirtuin 1 Cell Movement medicine Humans Protein kinase A Molecular Biology Cells Cultured Cell Proliferation biology Cell growth Tumor Necrosis Factor-alpha Cell Biology Cell biology Proliferating cell nuclear antigen MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis biology.protein Ectopic expression RNA Long Noncoding |
| Zdroj: | Journal of cellular biochemistry. 120(3) |
| ISSN: | 1097-4644 |
| Popis: | Deregulated proliferation of vascular smooth muscle cells (VSMCs) is one common phenomenon of atherosclerosis progression. Long noncoding RNAs (lncRNAs) are one group of noncoding RNAs that play essential roles in many cell biological processes, including cell development, growth, and migration. However, the role of a novel calcium/calmodulin-dependent protein kinase type II subunit delta (CAMK2D)-associated lncRNA, CAMK2D-associated transcript 1 (C2dat1), in VSMCs is still uncovered. In this study, we showed that the expression level of C2dat1 was higher in coronary artery disease (CAD) tissues than in normal arterial tissues and the C2dat1 expression level was upregulated in the proliferating VSMC after being treated with PDGF-bb or TNF-α. In addition, we indicated that overexpression of C2dat1 promoted VSMC growth and enhanced proliferating cell nuclear antigen (PCNA) expression in VSMC. Moreover, ectopic expression of C2dat1 increased VSMC migration. Furthermore, we showed that elevated expression of C2dat1 suppressed microRNA-34a (miR-34a) expression and promoted sirtuin 1 (SIRT1) expression, which was a direct target gene of miR-34a. We demonstrated that the expression level of miR-34a was lower in CAD tissues than in normal arterial tissues and the expression of miR-34a was negatively correlated with C2dat1 expression. Restored expression of C2dat1 increased VSMC proliferation and migration through promoting SIRT1 expression. These data suggested that lncRNA C2dat1 might be a potential therapeutic target to promote VSMC growth and migration in CAD. |
| Databáze: | OpenAIRE |
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