A sensitive method for digoxin determination using formate-adduct ion based on the effect of ionization enhancement in liquid chromatograph–mass spectrometer
| Autor: | Xia Li, Yu Wang, Lihong Chen, Jie Zheng, Yunqiu Yu, Qing-Yuan Zhou |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Digoxin
Formates Formic acid Calibration curve Digitoxin Clinical Biochemistry Mass spectrometry Sensitivity and Specificity Biochemistry Analytical Chemistry chemistry.chemical_compound Drug Stability Tandem Mass Spectrometry Deslanoside medicine Animals Humans Formate Detection limit Chromatography Reproducibility of Results Cell Biology General Medicine Rats chemistry Linear Models Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B. :138-144 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2014.11.023 |
| Popis: | A sensitive and rapid method based on formate-adduct ion detection was developed and fully validated for digoxin determination in rat plasma. For LC/MS/MS detection with formate-adducts as precursor ions, transitions of m/z 825.5→779.9 for digoxin and m/z 809.5→763.4 for the internal standard (digitoxin) were monitored in negative mode. To investigate the impact of formic acid on the mass response and method sensitivity, a formic acid concentration range of 0-0.1% (0, 0.0005%, 0.002%, 0.01%, 0.1%, v/v) was evaluated. A concentration of 0.002% gave the highest sensitivity, which was 16- to 18-fold higher than deprotonated ions, and was designated as the contribution giving the strongest ionization enhancement and adduction. A number of parameters were then varied in order to optimize the method, and a limit of quantitation (LOQ) at 0.2 ng/mL was reached with an injection volume of 5 μL, a total run time of 3 min, and 0.1 mL of rat plasma. A calibration curve was plotted over the range 0.2-50 ng/mL (R(2)=0.9998), and the method was successfully applied to study pharmacokinetics in rat following a single oral administration of digoxin (0.05 mg/kg). Four additional steroid saponins (digitoxin, deslanoside, ginsenoside Rg1 and Rb1) were investigated to assess the impact of formic acid on the mass response of steroid saponins. Compounds with a conjugated lactonic ring in their structures such as digoxin, digitoxin and deslanoside tended to form stable formate-adduct ions more easily. The LC/MS/MS method developed here is therefore well suited for the quantification of steroid saponins that are difficult to deprotonate using other MS approaches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect