Determining the protein drug release characteristics and cell adhesion to a PLLA or PLGA biodegradable polymer membrane
| Autor: | Wesley L. Hicks, Joseph A. Gardella, David M. Cohan, Robert Hard, Sarah A. Burns, Lynn Sigurdson, Frank V. Bright |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Fibroblast Growth Factor 7 Biocompatibility Polymers Polyesters Biomedical Engineering Biocompatible Materials Cell Line Biomaterials chemistry.chemical_compound Drug Delivery Systems Pulmonary surfactant Polylactic Acid-Polyglycolic Acid Copolymer Polymer chemistry Materials Testing Cell Adhesion Humans Lactic Acid Drug Carriers Metals and Alloys Biomaterial Controlled release Biodegradable polymer PLGA Membrane chemistry Ceramics and Composites Biophysics Drug carrier Polyglycolic Acid |
| Zdroj: | Journal of biomedical materials research. Part A. 94(1) |
| ISSN: | 1552-4965 |
| Popis: | Biodegradable polymers are of interest for developing controlled protein drug delivery platforms. In this study, two poly (alpha-hydroxy) esters were formulated with Aerosol-OT, a surfactant stabilizer, to encapsulate the protein keratinocyte growth factor (KGF) for controlled release KGF is involved in a number of crucial biologic processes, most notably epithelial growth and repair. The concentration of KGF that caused a biological response in vitro was determined (optimally 10 ng/mL) and compared with the release of KGF from the two biodegradable polymer membrane formulations. Each polymer formulation released biologically relevant levels, 10 ng/mL of active KGF, although with different times release kinetics. The membrane composed of PLGA/AOT/KGF exhibited a faster release rate of KGF into solution after 120 h of degradation time than the release rate of the PLLA/AOT/KGF matrices. Cell seeding assays showed that both polymer matrices, when formulated with AOT, sustained cell growth. Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) was used to characterize the distribution of AOT and KGF through the polymer membrane. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010. |
| Databáze: | OpenAIRE |
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