Effect of Intrarectal Prostaglandin E2Analogue (Enprostil) on Trinitrobenzenesulphonic Acid-Induced Colitis in Rats
| Autor: | Kunihiko Murase, Kazuya Makiyama, Kohno S, Hajime Isomoto, Hisashi Furusu, Y. Mizuta, F Takeshima, Yasunori Onizuka |
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| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Colon medicine.medical_treatment 030204 cardiovascular system & hematology Biochemistry Gastroenterology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Enprostil Internal medicine medicine Animals Intestinal Mucosa Colitis Prostaglandin E2 Proctitis Chemotherapy business.industry Biochemistry (medical) Cell Biology General Medicine Enema medicine.disease Rats Thromboxane B2 Trinitrobenzenesulfonic Acid chemistry 030220 oncology & carcinogenesis Rectal administration Eicosanoids business medicine.drug |
| Zdroj: | Journal of International Medical Research. 28:28-35 |
| ISSN: | 1473-2300 0300-0605 |
| DOI: | 10.1177/147323000002800105 |
| Popis: | Prostaglandins exert a protective effect on colonic mucosa in experimentally induced colitis. This study investigated the effect of enprostil, a prostaglandin E2(PGE2) analogue, on trinitrobenzenesulphonic acid (TNBS)-induced colitis in rats. Each rat received a rectal enema containing TNBS (30 mg), followed 24 h later by intrarectal once-daily enprostil (200 μg). Enprostil-treated and control rats were killed on day 3 (enprostil group, n = 5; control, n = 6) or day 10 (enprostil group, n = 5; control, n = 5) after TNBS treatment. The area of damaged mucosa of the colon was measured relative to the total colonic area. We also determined the macroscopic score of mucosal damage, and measured PGE2, 6-ketoprostaglandin F1α(6-keto-PGF1α) and thromboxane B2(TXB2) concentration in portal vein blood samples. Enprostil significantly reduced both the area of damaged mucosa (including the ulcer area) and the macroscopic score after 3 days' treatment compared with control. Similarly, enprostil significantly reduced plasma concentration of PGE2, 6-keto-PGF1αand TXB2during the acute phase at day 3 of treatment compared with control, but not at day 10. These results suggest that PGE2enema may have therapeutic potential for treating patients with proctitis or left-sided colitis. |
| Databáze: | OpenAIRE |
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