A bioluminescent reporter for the halophilic archaeon Haloferax volcanii
| Autor: | Chris R. Davis, J. Brian Robertson, Carl Hirschie Johnson |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Salinity
0303 health sciences biology 030306 microbiology Chemistry Haloferax volcanii General Medicine biology.organism_classification Microbiology Article Halophile Luminescent Proteins 03 medical and health sciences Biochemistry Halophilic archaeon Genes Reporter Proper function Haloarchaea Molecular Medicine Bioluminescence Luciferase Function (biology) 030304 developmental biology |
| Zdroj: | Extremophiles |
| ISSN: | 1433-4909 1431-0651 |
| DOI: | 10.1007/s00792-020-01193-x |
| Popis: | Haloarchaea have evolved to thrive in hypersaline environments. Haloferax volcanii is of particular interest due to its genetic tractability; however, few in vivo reporters exist for halophiles. Haloarchaeal proteins evolved characteristics that promote proper folding and function at high salt concentrations, but many mesophilic reporter proteins lack these characteristics. Mesophilic proteins that acquire salt-stabilizing mutations, however, can lead to proper function in haloarchaea. Using laboratory-directed evolution, we developed and demonstrated an in vivo luciferase that functions in the hypersaline cytosol of H. volcanii. |
| Databáze: | OpenAIRE |
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