Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)
| Autor: | Juan-Antonio Sanchez, Thomas Van Leeuwen, Mauro Nannini, Tom Van de Wiele, Thijs Machtelinckx, Winnok H. De Vos, Patrick De Clercq, Godelieve Gheysen, Nico Boon |
|---|---|
| Přispěvatelé: | Evolutionary Biology (IBED, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Microbiology (medical) Adalia bipunctata Gene Transfer Horizontal RICKETTSIA WOLBACHIA BACTERIA ADALIA-BIPUNCTATA Population lcsh:QR1-502 Zoology Microbiology lcsh:Microbiology Hemiptera RNA Ribosomal 16S GRADIENT GEL-ELECTROPHORESIS BACTERIAL SYMBIONT Animals Rickettsia education CYTOPLASMIC INCOMPATIBILITY In Situ Hybridization Fluorescence Phylogeny education.field_of_study biology PARASITIC WASP Sequence Analysis RNA Host (biology) NONCULTIVATED PLANTS Research Microbiota 16S RIBOSOMAL-RNA Ovary Biology and Life Sciences biochemical phenomena metabolism and nutrition biology.organism_classification Miridae RNA Bacterial BACTEROIDETES SYMBIONT bacteria Female Wolbachia Cytoplasmic incompatibility |
| Zdroj: | BMC Microbiology, 12(suppl. 1). BioMed Central BMC MICROBIOLOGY BMC Microbiology BMC Microbiology, Vol 12, Iss Suppl 1, p S9 (2012) |
| ISSN: | 1471-2180 |
| Popis: | Background The predatory mirids of the genus Macrolophus are key natural enemies of various economically important agricultural pests. Both M. caliginosus and M. pygmaeus are commercially available for the augmentative biological control of arthropod pests in European greenhouses. The latter species is known to be infected with Wolbachia -inducing cytoplasmic incompatibility in its host- but the presence of other endosymbionts has not been demonstrated. In the present study, the microbial diversity was examined in various populations of M. caliginosus and M. pygmaeus by 16S rRNA sequencing and denaturing gradient gel electrophoresis. Results Besides Wolbachia, a co-infection of 2 Rickettsia species was detected in all M. pygmaeus populations. Based on a concatenated alignment of the 16S rRNA gene, the gltA gene and the coxA gene, the first is phylogenetically related to Rickettsia bellii, whereas the other is closely related to Rickettsia limoniae. All M. caliginosus populations were infected with the same Wolbachia and limoniae-like Rickettsia strain as M. pygmaeus, but did not harbour the bellii-like Rickettsia strain. Interestingly, individuals with a single infection were not found. A PCR assay on the ovaries of M. pygmaeus and M. caliginosus indicated that all endosymbionts are vertically transmitted. The presence of Wolbachia and Rickettsia in oocytes was confirmed by a fluorescence in situ hybridisation. A bio-assay comparing an infected and an uninfected M. pygmaeus population suggested that the endosymbionts had minor effects on nymphal development of their insect host and did not influence its fecundity. Conclusion Two species of the palaearctic mirid genus Macrolophus are infected with multiple endosymbionts, including Wolbachia and Rickettsia. Independent of the origin, all tested populations of both M. pygmaeus and M. caliginosus were infected with three and two endosymbionts, respectively. There was no indication that infection with endosymbiotic bacteria had a fitness cost in terms of development and fecundity of the predators. |
| Databáze: | OpenAIRE |
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