Glucose tolerance and plasma insulin response to intravenous glucose infusion and test meal in rats with microencapsulated islet allografts

Autor: J.H. Strubbe, Wm Fritschy, R van Schilfgaarde, G. H. J. Wolters
Rok vydání: 1991
Předmět:
Blood Glucose
Male
Time Factors
Endocrinology
Diabetes and Metabolism
Islets of Langerhans Transplantation
Dosage form
Eating
Insulin Secretion
IMPLANT
Insulin
MICROENCAPSULATION
Glucose tolerance test
geography.geographical_feature_category
medicine.diagnostic_test
ISLET TRANSPLANTATION
Islet
medicine.anatomical_structure
STREPTOZOTOCIN-DIABETIC RATS
GLUCOSE TOLERANCE
SURVIVAL
DIABETIC RATS
Pancreas
medicine.drug
medicine.medical_specialty
STREPTOZOTOCIN
Diabetes Mellitus
Experimental
Internal medicine
Diabetes mellitus
Internal Medicine
medicine
Animals
Transplantation
Homologous
KINETICS
RELEASE
PANCREAS
geography
INVITRO
business.industry
TRANSPLANTATION
Body Weight
Rats
Inbred Strains
Glucose Tolerance Test
medicine.disease
Streptozotocin
Rats
Transplantation
MICE
Endocrinology
Concomitant
business
Zdroj: Diabetologia, 34(8), 542-547. SPRINGER
ISSN: 0012-186X
Popis: Albino Oxford rats made diabetic with 75 mg/kg streptozotocin were intraperitoneally transplanted with 2500-2900 alginate-polylysine microencapsulated Lewis islets (n = 9, total islet tissue volume 8.0-11.0 microliters), or a similar volume of non-encapsulated Lewis islets (n = 5). All rats with microencapsulated islets became normoglycaemic, and remained normoglycaemic for 5-16 weeks. In rats with non-encapsulated islet grafts, only a temporary decrease in blood glucose was observed, and all were again severely hyperglycaemic at 1 week after implantation. At 5-6 weeks after transplantation, glucose tolerance in rats with microencapsulated islets was tested by intravenous glucose infusion (10 mg/min over 20 min) and test meal administration (n = 4). During glucose infusion, maximum glucose levels were 13.0 +/- 0.4 mmol/l in rats with microcapsules and 8.9 +/- 0.4 mmol/l in healthy control rats (p less than 0.01). Concomitant maximum plasma insulin levels were 215 +/- 17 pmol/l in rats with microcapsules and 715 +/- 85 pmol/l in controls (p less than 0.001). After the test meal, maximum blood glucose was 10.6 +/- 0.9 mmol/l in rats with microcapsules and 6.2 +/- 0.1 mmol/l in controls (p less than 0.001), with concomitant maximum plasma insulin levels of 247 +/- 11 pmol/l and 586 +/- 59 pmol/l, respectively (p less than 0.001). In conclusion, although the glucose tolerance is impaired and plasma insulin responses to intravenous glucose-load and test-meal are reduced, the alginate-polylysine membrane does provide adequate immunoisolation for the prolongation of allograft survival, resulting in prolonged normoglycaemia in streptozotocin diabetic rats.
Databáze: OpenAIRE
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